痹通冲剂中羟基红花黄色素A和葛根素的含量测定  

Content Determnation of Hydroxysafflor Yellow A and Puerarin in Bitong Granules

作　　者：许爱珍[1] 李欢 张宏[1] 张悦 石贵荣[1] 安明[2] 张耀升 郭晓文 XU Aizhen;LI Huan;ZHANG Hong;ZHANG Yue;SHI Guirong;AN Ming;ZHANG Yaosheng;GUO Xiaowen(Dept.of Preparation,Baotou Centre Hospital,Inner Mongolia Baotou 014040,China;College of Pharmacy,Baotou Medical College,Inner Mongolia Baotou 014040,China)

机构地区：[1]包头市中心医院制剂科,内蒙古包头014040 [2]包头医学院药学院,内蒙古包头014040

出　　处：《中国医院用药评价与分析》2019年第6期721-723,728,共4页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的:建立同时测定痹通冲剂中羟基红花黄色素A、葛根素2种成分含量的高效液相色谱法。方法:色谱柱为安捷伦C18柱(250mm×4.6mm,5.0μm);流动相为乙腈(A)和0.1%磷酸水溶液(B),梯度洗脱,流速为1.0ml/min;柱温为35℃;检测波长为253nm。结果:羟基红花黄色素A、葛根素含量分别在19.45~97.25μg/ml(r=0.99996)、23.30~116.50μg/ml(r=0.99998)范围内与峰面积呈良好的线性关系;羟基红花黄色素A的加样回收率为90.36%~103.6%,RSD为2.31%(n=9);葛根素的加样回收率为97.14%~109.24%,RSD为1.25%(n=9)。3批供试品中羟基红花黄色素A、葛根素的含量分别为0.8586、1.4605mg/g。结论:本研究建立的方法简便、快速且准确,可用于痹通冲剂的质量控制。OBJECTIVE: To establish the method for content determination of hydrosysafflor yellow A and puerarin at the same time in Bitong granules by high performance liquid chromatography(HPLC). METHODS: The chromatographic column was agilent C18(250 mm×4.6 mm, 5 μm), the mobile phases was acetonitrile(A) and 0.1% aqueous phosphoric acid(B)(gradient elute), with flow rate of 1.0 ml/min, column temperature of 35 ℃, and detective wavelength of 253 nm. RESULTS: The calibration curve of hydroxysafflor yellow A was linear over the range of 19.45 μg/ml to 97.25 μg/ml(r=0.999 96), the calibration curve of puerarin was linear over the range of 23.30 μg/ml to 116.50 μg/ml(r=0.999 98). The recovery rate of hydroxysafflor yellow A was 90.36% to 103.6%, RSD was 2.31%(n=9), the recovery rate of puerarin was 97.14% to 109.24%, RSD was 1.25%(n=9). The average content of hydroxysafflor yellow A was 0.858 6 mg/g and the average content of puerarin was 1.460 5 mg/g in three batches of Bitong granules. CONCLUSIONS: The method established in this study is simple, rapid and accurate, and can be used for quality control of Bitong granules.

关 键 词：痹通冲剂 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]