乙醇雾化对金黄色葡萄球菌肺炎大鼠炎症反应的调控作用  

Regulation of Ethanol Atomization on Inflammatory Response in Rats with Staphylococcus Aureus Pneumonia

作　　者：尚莉莉[1] 刘凯[1] 全爱君 蔡国锋[1] 梅花·尼合买提 胡才宝[3] SHANG Lili;LIU Kai;QUAN Aijun;CAI Guofeng;Meihua·NI KOMTI;HU Caibao(1.Intensive Care Unit,the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Heilongjiang Harbin 150001,China;Dept.of Internal Medicine,Kazakh Hospital in Altay Area of Xinjiang Uygur Autonomous Region,Xinjiang Altay 836500,China;Intensive Care Unit,Zhejiang Hospital,Zhejiang Hangzhou 310013,China)

机构地区：[1]黑龙江中医药大学附属第二医院重症医学科,黑龙江哈尔滨150001 [2]新疆维吾尔自治区阿勒泰地区哈萨克医院内科,新疆阿勒泰836500 [3]浙江医院重症医学科,浙江杭州310013

出　　处：《中国医院用药评价与分析》2019年第7期811-814,共4页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：黑龙江省自然科学基金项目(No.H2016053)

摘　　要：目的:探讨金黄色葡萄球菌肺炎大鼠雾化吸入不同浓度乙醇后,支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)细胞分类计数、肺组织中炎性因子的含量及炎性因子细胞通路相关基因表达的变化,以期为乙醇治疗肺炎提供参考.方法:采用10%、40%和70%的乙醇对感染金黄色葡萄球菌肺炎的大鼠进行雾化治疗,对BALF中的细胞进行分类计数,采用酶联免疫吸附法测定大鼠肺组织匀浆中白细胞介素(IL)1、IL-6、IL-8、IL-10、γ-干扰素(INF-γ)及肿瘤坏死因子α(TNF-α)的含量,采用荧光定量聚合酶链式反应法测定大鼠肺组织Toll样受体4(Toll-like receptor 4,TLR4)、髓样分化因子88(myeloid differentiation factor 88,MyD88)、肿瘤坏死因子受体相关因子6(tumor necrosis factor receptor-associated factor 6,TRAF6)、TANK结合激酶1(TANK-binding kinase 1,TBK1)和核因子κB(nuclear factor kappa B,NF-κB)基因的相对表达量.结果:随着乙醇浓度的升高,细菌计数呈降低趋势,细胞总数、巨噬细胞数及中性粒细胞数呈降低趋势,炎性因子含量呈现先降低后升高的趋势,各目的基因的相对表达量也呈现先降低后升高的趋势.10%乙醇的效果并不显著,而70%乙醇又会对正常肺泡细胞造成损伤,40%乙醇治疗后各项指标与对照组最为接近.结论:乙醇雾化吸入可治疗大鼠的金黄色葡萄球菌肺炎,以40%乙醇对炎症反应的调控效果最理想.OBJECTIVE:To probe into the changes of cell classification count of bronchoalveolar lavage fluid(BALF),the content of inflammatory factors in lung tissues and the expression of genes related to inflammatory factor cell pathway after inhalation of different concentrations of ethanol in rats with Staphylococcus aureus pneumonia,so as to provide reference for ethanol treatment of pneumonia.METHODS:In this study,rats infected with S.aureus pneumonia were atomized with ethanol in concentration of 10%,40%and 70%.Classification and counting of cells in BALF were researched.IL-1,IL-6,IL-8,IL-10,INF-γ,and TNF-αin rat lung homogenate were determined by ELISA.The relative expression levels of TLR4,MyD88,TRAF6,TBK1 and NF-κB genes in lung tissues of rats were determined by real-time quantitative PCR.RESULTS:With the increase of ethanol concentration,the bacterial count decreased,the total number of cells,the number of macrophages and the number of neutrophils decreased,and the level of inflammatory factors first decreased and then increased,and the relative expression levels of various target genes also showed a decreasing trend first and then increasing trend.The effect of 10%ethanol was not significant,while 70%ethanol caused damage to normal alveolar cells,and 40%ethanol was the closest to the control group after treatment.CONCLUSIONS:Ethanol atomization can intreatment of S.aureus pneumonia,and 40%ethanol has the best effect on the regulation of inflammation.

关 键 词：金黄色葡萄球菌 肺炎 乙醇 雾化 炎性因子 

分 类 号：R96[医药卫生—药理学]