2018年临沂市MSM人群新感染 HIV-1病毒基因亚型分析  

Analysis on virus genotypes of new infected HIV-1 among MSM,Linyi city,2018

作　　者：季圣翔[1] 张晓妮 尹德清[1] 苏生利[2] JI Sheng-xiang;ZHANG Xiao-ni;YIN De-qing;SU Sheng-li(Linyi City Center for Disease Control and Prevention LinyiShandong,276000 ,China)

机构地区：[1]临沂市疾病预防控制中心,山东临沂276000 [2]山东省疾病预防控制中心,山东济南250014

出　　处：《预防医学论坛》2019年第6期411-413,共3页Preventive Medicine Tribune

基　　金：临沂市重点研发计划项目(2018YD015)

摘　　要：目的了解临沂市MSM人群新感染HIV-1病毒基因的流行亚型。方法收集2018年1~4月临沂市新感染的经男男性行为传播的未经治疗的HIV-1阳性血浆样本,应用巢式-聚合酶链反应(Nested-PCR)扩增病毒基因的pol区片段,进行基因测序和亚型分析。结果收集到符合条件的样本31例,成功扩增出29例感染者病毒的有效序列,共检出4种基因亚型:CRF07_BC、CRF01_AE、C、CRF55_01B,优势毒株为CRF01_AE(62.07%)和CRF07_BC(20.69%)亚型。结论本时段内临沂市新确证的MSM感染者毒株以CRF01_AE亚型为主,监测临沂市MSM人群HIV-1感染者毒株亚型分子流行病学特征,对临沂市艾滋病防治非常必要。Objective To understand the epidemic subtypes of HIV-1 among newly HIV-infected MSM in Linyi city. Methods Plasma samples were collected from newly diagnosed HIV-1 MSM in Linyi city from January to April 2018.The study was performed on pol subgenomic regions amplified by Nestd-PCR,and characterized by sequence analysis. Results A total of 31 eligible samples were collected and pol gene fragments of HIV-1 were successfully amplified in 29 samples,4 subtypes were detected:CRF07BC CRF01AE,C,CRF5501 B,the dominant strains were CRF01AE(62.07%) and CRF07BC(20.69%) subtypes. Conclusion CRF01AE subtype is predominant in MSM infection cases in Linyi city during this study period.It is necessary to monitor the molecular epidemiological characteristics of HIV-1 MSM in Linyi city for the prevention and treatment of AIDS.

关 键 词：男男同性性行为 人类免疫缺陷病毒 亚型 

分 类 号：R512.91[医药卫生—内科学]