御唐丸对糖尿病大鼠胰腺组织JNK、Akt基因及蛋白表达和磷酸化修饰的影响  

JNK and Akt genes in pancreatic tissue of diabetic rats and the effects of protein expression and phosphorylation modification

作　　者：郑南 谢宁[1] 张佩青 高丽娟[3] Zheng Nan;Xie Ning;Zhang Peiqing;Gao Lijuan(Basic Medical College, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China;Department of Kidney Diseases, Heilongjiang Academy of Chinese Medicine, Harbin 150000, China;Rheumatology Department, First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China)

机构地区：[1]黑龙江中医药大学基础医学院,哈尔滨150040 [2]黑龙江省中医药科学院肾病科,哈尔滨150000 [3]黑龙江中医药大学附属第一医院风湿科,哈尔滨150040

出　　处：《国际中医中药杂志》2019年第7期723-727,共5页International Journal of Traditional Chinese Medicine

基　　金：教育部春晖计划资助项目(5030411);黑龙江省博士后资助项目(LBH-Z17206);黑龙江中医药大学博士创新基金(2017bs03).

摘　　要：目的观察御唐丸对糖尿病大鼠胰腺组织c-Jun氨基末端激酶(c-Jun n-terminal kinase, JNK)、蛋白激酶B(protein kinase B, Akt)基因及蛋白表达和磷酸化修饰的影响。方法将大鼠按随机数字表法分为空白组、模型组、西药对照组、中药对照组、御唐丸低剂量组、御唐丸高剂量组,每组20只。除空白组外,其余各组大鼠制备糖尿病模型。西药对照组灌胃盐酸二甲双胍片混悬液0.09 g/kg,中药对照组灌胃糖尿乐片混悬液2.43 g/kg,御唐丸低、高剂量组分别灌胃御唐丸1.35、2.70 g/kg,空白组和模型组灌胃等体积生理盐水。1次/d,连续给药8周后取材。采用Real-time PCR方法检测胰腺组织JNK、Akt基因表达水平;采用Western blot法检测胰腺组织JNK、Akt蛋白表达及其磷酸化水平。结果与模型组比较,御唐丸高、低剂量组JNK mRNA[(0.57±0.07)、(0.95±0.13)比(1.14±0.19)]、P-JNK/JNK [(0.222±0.038)、(0.817±0.104)比(1.140±0.136)]比值降低(P<0.01);御唐丸高剂量组Akt mRNA [(2.09±0.13)比(1.63±0.12)]表达升高,P-Akt(Ser473)/Akt[(0.385±0.072)比(0.130±0.027)]比值升高(P<0.01)。结论御唐丸可下调T2DM大鼠胰腺组织JNK mRNA的表达,抑制JNK蛋白磷酸化水平;上调Akt mRNA的表达,提高Akt蛋白磷酸化水平。Objective To observe the effects of Yutang pill on pancreatic tissue, JNK, Akt gene, protein expression and phosphorylation of diabetic rats. Methods A total of 150 male SD rats were divided into blank group, model group, western medicine control group, Chinese medicine control group, Yutang Pill low dose group and Yutang pill high dose group according to random number table, 20 rats in each group. In addition to the blank group, the other groups of rats were prepared for the diabetes model. The western medicine control group was intragastrically administered with metformin hydrochloride suspension 0.09 g/kg, the Chinese medicine control group was administered with the suspension of glycoside tablets 2.43 g/kg, and the low-dose and high-dose groups of Yutang pills were respectively administered with Yutang pills 1.35 and 2.70 g/kg. The blank group and the model group were intragastrically administered with equal volume of saline. 1 time/d, 8 weeks. After continuous administration, the material was taken. The expression levels of JNK and Akt in pancreatic tissue were detected by Real-time PCR. The expression of JNK and Akt protein and phosphorylation in pancreatic tissue were detected by Western blot. Results Compared with the model group, the expression of JNK mRNA (0.57 ± 0.07, 0.95 ± 0.13 vs. 1.14 ± 0.19) and P-JNK/JNK (0.222 ± 0.038, 0.817 ± 0.104 vs. 1.140 ± 0.136) in the low- and high dose group significantly decreased (P<0.01). The expression of Akt mRNA (2.09 ± 0.13 vs. 1.63 ± 0.12) and P-Akt(Ser473)/ Akt (0.385 ± 0.072 vs. 0.130 ± 0.027) significantly increased (P<0.01). Conclusions The Yutang pill can reduce the expression of JNKmRNA in pancreatic tissue of T2DM rats and inhibit the phosphorylation of JNK protein. The expression of AktmRNA increased and the phosphorylation level of Akt protein increased.

关 键 词：糖尿病 御唐丸 C-JUN氨基末端激酶 蛋白激酶B 大鼠 

分 类 号：R285.5[医药卫生—中药学]