模拟失重对成年小鼠闪光视网膜电图明视负波反应的影响  

Effect of simulated microgravity on the photopic negative response of flash electroretinogram in adult mice

作　　者：戴旭锋[1] 保金华[1] 陈晓萍[2] 黄海笑[1] 李文炯[2] 陈浩[1] Dai Xufeng;Bao Jinhua;Chen Xiaoping;Huang Haixiao;Li Ivenjiong;Chen Hao(School of Optometry and Ophthalmology, Wenzhou Medical University, Wenzhou 325027, China;National Key Laboratory of Human Factors Engineering, China Astronaut Research and Training Center, Beijing 100094,China)

机构地区：[1]温州医科大学眼视光学院,325027 [2]中国航天员科研训练中心人因工程重点实验室,北京100094

出　　处：《中华眼底病杂志》2019年第4期379-384,共6页Chinese Journal of Ocular Fundus Diseases

基　　金：载人航天第三批预先研究基金(020201 );浙江省自然科学基金(LY15H120002).

摘　　要：目的观察模拟失重对成年小鼠闪光ERG明视负波反应(PhNR)的影响。方法实验研究。正常成年雄性C57BL/6J小鼠48只随机分成模拟失重组和对照组,每组再分为A、B、C 3个亚组,各亚组8只小鼠。模拟失重组A、B亚组悬尾时间分别为15、30 d,悬尾时间达到要求后,马上对观察指标进行检测;模拟失重组C亚组悬尾30 d,在检测指标前先恢复正常体位30 d。对照组A、B、C亚组小鼠分别保持正常体位15、30、60 d,然后同对应天数的模拟失重亚组一起进行实验指标的检测。行闪光ERG检查,以基线到波谷为PhNR振幅并记录;行闪光VEP检查,记录N1波峰时和P1-N1振幅。行水中逃生实验,根据小鼠逃生时间量化评估小鼠整体视功能状态。行OCT检查,测量小鼠视网膜内层厚度。行视网膜石蜡切片,观察小鼠视网膜结构。模拟失重组与对照组各亚组数据比较采用独立样本 t 检验。结果模拟失重组A亚组PhNR振幅较对照组A亚组明显降低,差异有统计学意义(t=?3.196,P<0.01);模拟失重组B、C亚组分别与对照组B、C亚组PhNR振幅比较,差异均无统计学意义(t=?1.976、0.285,P>0.05)。模拟失重组A、B、C亚组与对照组A、B、C亚组的N1波峰时、P1-N1振幅比较,差异均无统计学意义(P>0.05)。模拟失重组A、B、C亚组与对照组A、B、C亚组视网膜内层厚度比较,差异均无统计学意义(t=?0.461、2.073、?0.402,P>0.05)。模拟失重组各亚组小鼠RGC层、内丛状层、内核层、外丛状层、外核层、椭圆体带和RPE等结构均未见异常。模拟失重组A、B、C亚组与对照组A、B、C亚组小鼠逃生时间比较,差异均无统计学意义(t=?0.637、?0.955、1.297,P>0.05)。结论短期模拟失重对小鼠闪光ERG的PhNR可能产生暂时的一定程度的抑制作用,但并不影响整体视功能。Objective To observe the effect of simulated microgravity on the photopic negative response (PhNR) of full-field flash ERG in adult mice. Methods In an experimental study, forty-eight adult male C57BL/6J mice (48 eyes) were randomly divided into model and control groups. Model mice were further divided into three subgroups of 8 each: tail-suspended for 15 days (subgroup A), tail-suspended for 30 days (subgroup B), and tail-suspended for 30 days followed by returning to normal position for 30 days (subgroup C). The three control subgroups were similarly fixed with a harness but kept in the normal position for corresponding periods of 15, 30, and 60 days. The mice were immediately examined using ERG-PhNR, flash VEP, OCT and visually-guided behavior in vivo, and subsequently sacrificed to analyze the retinal histology in vitro. PhNR amplitude was measured from baseline to PhNR trough. N1 peak-time and N1?P1 amplitude of VEP was analyzed. The escape duration was used to quantitatively evaluate the visual function of mice. In addition, inner retinal thickness was analyzed by OCT imaging. Data were compared by the independent sample /-test.Results PhNR amplitude in the model subgroup A was obviously lower than the corresponding control subgroup, the difference was statistically significant (/=-3」96, P<0.01). There was no significant difference in PhNR amplitude between the model subgroup B or C and the conesponding control subgroup (/=-1.976, 0.285;P>0.05). There was no significant difference in FVEP N1 peak-time or Nl-Pl amplitude between any of the three model subgroups and the corresponding control subgroup (P>0.05). There was no significant difference in OCT-measured inner retinal thickness between any of the three model subgroups and the corresponding control subgroup (/=-0.461, 2.073,-0.402;P>0.05). The three model subgroups showed almost normal retinal structure, including the retinal ganglion cell, inner pexiform layer, inner nuclear layer, outer plexifdrm layer, outer nuclear layer, ellipsoid zone and RPE. T

关 键 词：失重模拟 视网膜电描记术 动物实验 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]