三七总皂苷在乙酸所致小鼠急性炎性内脏痛中的镇痛作用  

Analgesic Effect of Panaxnotoginsengsaponins on Acute Inflammatory Visceral Pain in Mice Induced by Acetic Acid

作　　者：李霞 董航 于江 Li Xia;Dong Hang;Yu Jiang(Jianghan University Affiliated Hospital,Hubei,Wuhan 430016,China.)

机构地区：[1]江汉大学附属医院

出　　处：《中国中医急症》2019年第7期1171-1173,1187,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：湖北省自然科学基金项目(2014FGB324)

摘　　要：目的分析三七总皂苷(PNS)在乙酸所致小鼠急性炎性内脏痛中的镇痛作用。方法选取成年雄性昆明小鼠120只,随机分为对照组(n=20只),生理盐水组(n=50只),三七总皂苷组(n=50只)。对照组常规条件下饲养,不做干预建立急性内脏痛模型;生理盐水组预先腹腔注射生理盐水,剂量为2.86 m L/kg;三七总皂苷组预先腹腔注射三七总皂苷7 mg/mL,同时两组间隔12 h注射1次,持续7 d,第8日腹腔注射1%乙酸(10 mg/kg),建立急性内脏痛模型,即刻中观察昆明小鼠扭体反应。结果乙酸腹腔注射后3 min左右产生行为学反应,生理盐水组在15~30 min后扭体反应达到峰值,三七总皂苷组在30~45 min出现反应高峰值;三七总皂苷组和生理盐水组比较,0~15、15~30、45~60、60~75、120~135 min后扭体反应明显减弱(P<0.05);生理盐水组、三七总皂苷组与对照组比较显著增加(P<0.05);三七总皂苷组GFAP表达显著低于生理盐水组(P<0.05)。结论乙酸所致小鼠急性炎性内脏痛模型预先经腹腔注射三七总皂苷可抑制脊髓胶质细胞的激活,进一步缓解急性炎性内脏痛。Objective:To investigate the analgesic effect of panaxnotoginsengsaponins(PNS)on acute inflammatory visceral pain in mice induced by acetic acid.Methods:120 adult male Kunming mice were randomly divided into the control group(n=20),the normal saline group(n=50),and PNS group(n=50).The control group was fed under routine conditions without intervention to establish acute visceral pain model;the saline group was given intraperitoneal injection of saline at a dose of 2.86 m L/kg;PNS group was given intraperitoneal injection of PNS 7 mg/mL beforehand.The two groups were injected once every 12 hours for 7 days.The acute visceral pain model was established by intraperitoneal injection of 1%acetic acid(10 mg/kg)on the 8 th day.The writhing reaction of Kunming mice was observed immediately.Results:The behavioral response occurred about 3 minutes after the intraperitoneal injection of acetic acid.The writhing reaction reached a peak after 15~30 minutes in the normal saline group,and the high peak of the reaction occurred in PNS group in 30~45 minutes.The reaction of the PNS group was significantly milder than that of the normal saline group at 0~15,15~30,45~60,60~75,120~135 minutes(P<0.05).The expression of GFAP in the normal saline group and PNS group was significantly higher than that in the control group(P<0.05),and that in PNS group was significantly lower than that in the normal saline group(P<0.05).Conclusion:Pre-intraperitoneal injection of PNS can suppress the activation of spinal glial cells and further alleviate acute inflammatory visceral pain in mice model with acute inflammatory visceral pain caused by acetic acid.

关 键 词：急性内脏痛 三七总皂苷 乙酸 扭体反应 小鼠 

分 类 号：R285.5[医药卫生—中药学]