高海拔环境下大鼠血浆、肺组织SOD活力和MDA含量及肺组织AngⅡ mRNA表达观察  

Changes of oxidative stress in rats at high altitude

作　　者：石雪峰[1] 解友邦[1] 苟三玉 蒋慧[1] 顾玉海[1] SHI Xuefeng;XIE Youbang;GOU Sanyu;JIANG Hui;GU Yuhai(Qinghai Provincial Peopled Hospital9 Xining 810007, China)

机构地区：[1]青海省人民医院

出　　处：《山东医药》2019年第21期42-45,共4页Shandong Medical Journal

基　　金：青海省自然科学基金资助项目(2017-ZJ-954Q)

摘　　要：目的观察高海拔环境下大鼠血浆、肺组织超氧化物歧化酶(SOD)活力、丙二醛(MDA)水平以及肺组织AngⅡmRNA表达的变化,并探讨其三者之间的相关性,为探讨急性肺损伤的机制提供理论依据。方法 56只Wistar大鼠中,低海拔组(海拔1 500 m) 8只、中海拔组(海拔2 260 m) 8只及高海拔组40只(海拔5 000 m),其中低海拔组在采购当日、中海拔组于采购后饲养30 d取血及肺组织;高海拔组饲养1、3、7、15、30 d取肺组织,其中饲养30 d时同时留取血样本。采用水溶性四氮唑(WST-1)法检测各组血浆SOD活力,低海拔组及高海拔组1、3、7、15、30 d大鼠肺组织SOD活力。用硫代巴比妥(TBA)比色法测定各组血浆MDA含量,低海拔组及高海拔组饲养1、3、7、15、30 d大鼠肺组织MDA含量。用qRT-PCR法检测肺组织AngⅡmRNA的表达。结果低、中、高海拔组血浆MDA含量分别为(2. 28±0. 68)、(2. 76±0. 34)、(10. 34±1. 19) nmol/m L,SOD活力分别为(19. 98±1. 57)、(19. 06±1. 24)、(10. 34±1. 19) U/m L,血浆MDA含量比较:低海拔组<中海拔组<高海拔组(P均<0. 01);血浆SOD活力比较:低海拔组>中海拔组>高海拔组(P均<0. 01)。低海拔组,高海拔组饲养1、3、7、15、30 d肺组织MDA含量分别为(1. 81±0. 24)、(2. 39±0. 35)、(2. 99±0. 34)、(3. 44±0. 30)、(4. 03±0. 88)、(4. 66±0. 74)nmol/mg prot,SOD活力分别为(439. 85±33. 80)、(554. 07±24. 59)、(486. 78±18. 78)、(403. 71±16. 57)、(341. 98±17. 89)、(295. 98±11. 08) U/mg prot,AngⅡmRNA相对表达量分别为0. 39±0. 12、0. 51±0. 27、0. 98±0. 18、1. 21±0. 38、1. 54±0. 24、1. 89±0. 87。高海拔组饲养1 d肺组织MDA含量及SOD活力高于低海拔组(P均<0. 05);与高海拔组饲养1 d相比,饲养3、7、15、30 d MDA含量升高,且随高海拔低氧环境时间延长,MDA含量呈逐渐升高趋势(P均<0. 05)。SOD活力在高海拔组饲养1 d升高,随高海拔暴露时间的延长其活力呈逐渐降低趋势(P均<0. 05)。与低海�Objective To study the changes of superoxide dismutase( SOD) and malondialdehyde( MDA) levels in the plasma and lung tissues of mice,and the expression of Ang Ⅱ in rat lung tissues under high altitude environment.Methods Fifty Wistar rats were randomly divide into 3 groups: the low altitude group( 8 mice,la group,an altitude of 1500 meters),medium altitude group( 8 mice,ma group,an altitude of 2 260 meters),and high altitude group( 40 mice,ha group,an altitude of 5 000 meters). The plasma and lung tissues were taken on the day of purchase in the la group,and the ma group on the 30 th day after purchase. The plasma was taken at 1,3,7,15,30 days after purchase( ha1,ha3,ha7,ha15,and ha30 groups) in the ha group,and we took the lung tissues at 30 days after purchase. WST-1 method was used to detect the activity of SOD;TBA method was used to detect the content of MDA;qRT-PCR was used to detect the expression of AngⅡ mRNA. Results The MDA content in plasma of the la,ma and ha groups were( 2. 28 ± 0. 68),( 2. 76 ± 0. 34),and( 10. 34 ± 1. 19) nmol/m L,respectively. The SOD activities in plasma of the la,ma and ha groups were( 19. 98 ± 1. 57),( 19. 06 ± 1. 24),and( 10. 34 ± 1. 19) U/m L,respectively. The MDA content in plasma was in the following order: la group < ma group < ha group( P < 0. 01),and the SOD activity in plasma: la group > ma group > ha group( P < 0. 01). The MDA content in lung tissues of the la,ha1,ha3,ha7,ha15 and ha30 groups were( 1. 81 ±0. 24),( 2. 39 ± 0. 35),( 2. 99 ± 0. 34),( 3. 44 ± 0. 30),( 4. 03 ± 0. 88),and( 4. 66 ± 0. 74) nmol/mg prot,the SOD activities were( 439. 85 ± 33. 80),( 554. 07 ± 24. 59),( 486. 78 ± 18. 78),( 403. 71 ± 16. 57),( 341. 98 ± 17. 89),and( 295. 98 ± 11. 08) U/mg prot,and the AngⅡ expression was 0. 39 ± 0. 12,0. 51 ± 0. 0. 27,0. 98 ± 0. 18,1. 21 ± 0. 38,1. 54 ± 0. 24,and 1. 89 ± 0. 87,respectively. The MDA content and SOD activity was higher in the ha1 group than in the la group( both P < 0. 05);compared with the ha1 group,MDA content increased

关 键 词：氧化应激 超氧化歧化酶 丙二醛 血管紧张素Ⅱ 高氧环境 

分 类 号：R563[医药卫生—呼吸系统]