iNOS调节Rab8参与肥胖诱导的胰岛素抵抗  

iNOS participates in obesity-induced insulin resistance by regulating Rab8

作　　者：李瀚琪 牛文彦 LI Han-qi;NIU Wen-yan(Department of Immunology,School of Basic Medical Sciences,Tianjin Medical University,Tianjin300070,China)

机构地区：[1]天津医科大学基础医学院免疫学系

出　　处：《天津医科大学学报》2019年第3期201-204,共4页Journal of Tianjin Medical University

基　　金：国家自然科学基金面上项目资助(81670731,81870547,81170740);天津市科委应用基础研究重点项目资助(15JCZDJC35500);天津市卫计委重点攻关项目资助(15KG102)

摘　　要：目的:探究诱导型一氧化氮合酶(iNOS)对Rab8的调节与胰岛素抵抗的关系。方法:用普通饮食和高脂饮食分别喂养野生型和iNOS-/-小鼠12周,在第12周进行腹腔注射葡萄糖耐量实验和胰岛素耐量实验,测定体质量、附睾脂肪质量,Western blot检测骨骼肌中Rab8蛋白表达。结果:高脂饮食诱发小鼠肥胖和胰岛素抵抗。相比于野生型小鼠,iNOS-/-小鼠的体质量、附睾脂肪质量均显著降低,分别降低10.36 g和0.29 g(均P<0.001),胰岛素抵抗有显著改善,而骨骼肌Rab8蛋白水平显著升高,增高2.14倍(P<0.000 1)。结论:iNOS可能通过调节Rab8参与高脂饮食诱导的胰岛素抵抗。Objective: To investigate the relationship between the regulation of Rab8 by iNOS and insulin resistance. Methods: Wild-type and iNOS-/-mice were fed with chow diet and high-fat diet for 12 weeks, respectively. Intraperitoneal glucose tolerance test(ipGTT) and insulin tolerance test(ITT) were performed separately at the12 th week. Body weight and epididymal adipose weight were measured. The protein level of Rab8 in skeletal muscle was detected by Western blot. Results: High-fat diet induced insulin resistance and obesity in mice. Compared with wild-type mice, iNOS-/-mice had significantly lower body weight, epididymal adipose weight decreased by10.36 g and 0.29 g(P <0.001), and insulin resistence was significantly improved while Rab8 protein levels in skeletal muscle were significantly increased, increased by 2.14 times. Conclusion: iNOS may regulates Rab8 in insulin resistance induced by high fat diet in mice.

关 键 词：INOS 胰岛素抵抗 Rab8 糖尿病 

分 类 号：R392.1[医药卫生—免疫学]