β-榄香烯对非小细胞肺癌A549细胞增殖的影响  

Effect of β-elemene on proliferation of non-small cell lung cancer A549 cells

作　　者：程吕欢[1] 邬海[1] 于新慧 胡欣春[1] CHENG LU-huan;WU Hai;YU Xin-hui;HU Xin-chun(Department of Chest Surgery,Chest Hospital of Jiangxi Province,Nanchang 330006,Jiangxi Province,China;School of Basic Medical Sciences,College Jiangxi Medical College, Nanchang University,Nanchang 330006,Jiangxi Province,China)

机构地区：[1]江西省胸科医院胸外科,江西南昌330006 [2]南昌大学江西医学院基础医学院,江西南昌330006

出　　处：《中国临床药理学杂志》2019年第13期1340-1342,共3页The Chinese Journal of Clinical Pharmacology

基　　金：江西省自然科学基金面上基金资助项目(20161BAB205275)

摘　　要：目的观察β-榄香烯对非小细胞肺癌(NSCLC) A549细胞增殖的影响及其作用机制。方法将NSCLC A549细胞分成2组:对照组和β-榄香烯组。对照组细胞不做任何处理,β-榄香烯组先用不同浓度的β-榄香烯处理48 d,用CCK8检测细胞增殖,计算IC50值;然后用接近IC50值的β-榄香烯处理A549细胞48 d。用流式细胞仪检测细胞周期,用缺口末端标记法(TUNEL)检测细胞凋亡,以免疫印迹实验检测蛋白激酶B(Akt)、肿瘤蛋白p53(p53)、周期蛋白依赖激酶抑制剂1A(p21)表达水平。结果β-榄香烯的IC50值为57. 52 ng·m L^-1。对照组和β-榄香烯组的细胞周期S期比例分别为(20. 2±3. 8)%和(29. 5±2. 9)%,组间比较差异有统计学意义(P <0. 05);对照组和β-榄香烯组的细胞凋亡率分别是(2. 9±1. 9)%和(11. 2±4. 2)%,组间比较差异有统计学意义(P <0. 05);对照组和β-榄香烯组Akt蛋白表达水平分别为1. 10±0. 25和0. 45±0. 15,p53蛋白表达水平分别为0. 87±0. 14和1. 54±0. 28,p21蛋白表达水平分别为0. 34±0. 10和0. 59±0. 14,β-榄香烯组与对照组相比,各项指标差异均有统计学意义(均P <0. 05)。结论β-榄香烯可以减弱Akt信号通路、活化调控A549细胞周期和凋亡,从而抑制细胞增殖。Objective To investigate the effect of β- elemene on the proliferation of non - small - cell lung cancer ( NSCLC ) line A549. Methods NSCLC A549 cells were divided into two groups: control group and β- elemene group. The cells in control group did not do any treatment.β- elemene group treated with different concentrations of β- elemene for 48 h. CCK8 was used to detect cell proliferation and IC50 value was calculated. Then A549 cells were treated with approximate IC50 concentration of β- elemene for 48 h. Cell cycle was measured by flow cytometry. Cell apoptosis was detected by TdT - mediated dUTP nick - end labeling. The expression levels of the Serine threonine protein kinase ( Akt),Tumor suppressor gene P5 ( p53),and Cyclin dependent kinase inhibitor 1A( p21) were determined by Western blot. Results The IC50 concentration of β- elemene was 57. 52 ng·mL^-1 . The cell cycle S phase ratios in control group and β- elemene group were ( 20. 2 ± 3. 8 )% and ( 29. 5 ± 2. 9 )%,respectively. Compared with control group,β- elemene group had a higher proportion of the S phase, the difference was statistically significant ( P < 0. 05 ). The apoptosis rates of the two groups were ( 2. 9 ± 1. 9)% and ( 11. 2 ± 4. 2)%,respectively,with significant difference( P < 0. 05). Akt protein expression in control group and β- elemene group were 1. 10 ± 0. 25 and 0. 45 ± 0. 15;p53 protein expression in the two groups were 0. 87 ± 0. 14 and 1. 54 ± 0. 28;p21 protein expression in the two groups were 0. 34 ± 0. 10 and 0. 59 ± 0. 14. Comparison between control group with β- elemene group,the difference of the factors were significantly changed ( all P < 0. 05). Conclusion The β- elemene can attenuate Akt activity,regualte cell cycle and apoptosis,and inhibit proliferation of NSCLC A549 cells.

关 键 词：榄香烯 非小细胞肺癌细胞 增殖 凋亡 

分 类 号：R28[医药卫生—中药学]