pH值对污泥热水解/厌氧消化过程抗性基因的影响  

Effect of pH Value on the Decline of Resistance Genes in Sludge during the Thermal Hydrolysis and Anaerobic Digestion Combined Process

作　　者：姜明吉 薛罡[1,2] 陈红 孙敏[1] 刁茹琦 于鑫 高品 JIANG Ming-ji;XUE Gang;CHEN Hong;SUN Min;DIAO Ru-qi;YU Xin;GAO Pin(School of Environmental Science and Engineering, Donghua University, Shanghai 201620, China;Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200000 , China;Jiangsu Tongyan Environmental Protection Science and Technology Co. Ltd.,Yancheng 224000, China)

机构地区：[1]东华大学环境科学与工程学院,上海201620 [2]上海污染控制与生态安全研究院,上海200000 [3]江苏同盐环保科技有限公司,江苏盐城224000

出　　处：《中国给水排水》2019年第13期16-22,共7页China Water & Wastewater

基　　金：国家自然科学基金资助项目(51878136、51878135、51508081);江苏省自然科学基金资助项目(BK20160431)

摘　　要：通过改变污泥热水解的初始pH值(3、7、11)考察四环素抗性基因(TC-ARGs,包括tetA、tetC、tetM、tetO和tetX)及16S rRNA基因的变化,包括在泥相和液相中各基因丰度总和的变化及占比情况。同时,探讨了将不同pH值下的热水解污泥加入到厌氧消化系统后,TC-ARGs和16S rRNA基因在泥相和液相的丰度变化。结果表明,在pH值=3下进行污泥热水解时,泥相中TC-ARGs和16S rRNA基因有最好的削减效果,且液相中基因丰度较低,而在pH值=11时去除效果最差,且大量的TC-ARGs被释放到液相中;同样地,在pH值=3时,热水解/厌氧消化系统中泥相和液相的TC-ARGs和16S rRNA基因有最好的削减效果,而在单独厌氧消化过程和pH值=11时的热水解/厌氧消化过程削减效果较差。可见,初始pH值是影响污泥热水解及后续厌氧消化过程中TC-ARGs和16S rRNA基因变化的重要因素。The abundance of tetracycline resistance genes ( TC-ARGs, including tetA, tetC, tetM , tetO and tetX) and 16S rRNA genes was observed when changing the initial pH value ( pH =3,7, 11), including the sum and proportion of genes in sludge phase and the liquid phase. Additionally, the fate of TC-ARGs and 16S rRNA genes in the sludge and liquid phase was also investigated when the thermal hydrolysis of sludge with different inititd pH values were added to the digestion system. The results showed that the best reduction of TC-ARGs and 16S rRNA genes was observed at pH of 3 both in the sludge phase and liquid phase during sludge thermal hydrolysis, however, the removal of TC-ARGs was the worst and there was a large amount TC-ARGs released to the liquid phase at pH of 11;Moreover, at pH of 3 , TC-ARGs and 16S rRNA genes in sludge and liquid phase had the greatest decline in thermal hydrolysis - anaerobic digestion system, but the poor ARG reduction was observed in the anaerobic digestion process alone and thermal hydrolysis 一 anaerobic digestion at pH of 11. It can be seen that the initial pH value is an important factor affecting ARGs and 16S rRNA genes changes in the thermal hydrolysis and subsequent anaerobic digestion of sludge.

关 键 词：四环素抗性基因 污泥热水解 PH值 厌氧消化 

分 类 号：TU992[建筑科学—市政工程]