芎芍胶囊药物组分协同降低RAW264.7源性泡沫细胞胆固醇含量及抗炎作用  

Synergistic effect of components of Xiongshao capsule on reducing cholesterol content and inflammation in foam cells derived from RAW264.7 cells

作　　者：梅俊 周庆兵[2] 徐浩[3] 徐凤芹[2] MEI Jun;ZHOU Qingbing;XU Hao;XU Fengqin(Graduate School, China Academy of Chinese Medical Sciences, Beijing 100700;Institute of Geriatrics, China Academy of Chinese Medical Sciences, Beijing 100091;Institute of Cardiovascular Disease, China Academy of Chinese Medical Sciences ,Beijing 100091)

机构地区：[1]中国中医科学院研究生院,北京市100700 [2]中国中医科学院老年医学研究所,北京市100091 [3]中国中医科学院心血管病研究所,北京市100091

出　　处：《中国动脉硬化杂志》2019年第8期655-660,共6页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(81473529);中国中医科学院自主课题项目(220808043)

摘　　要：目的从体外细胞实验的角度,观察芎芍胶囊药物组分对泡沫细胞内胆固醇及炎症因子的影响作用。方法以氧化型低密度脂蛋白(ox-LDL)诱导RAW264.7细胞泡沫化;以CCK-8试剂检测泡沫细胞增殖活性;以胆固醇检测试剂盒检测泡沫细胞内总胆固醇(TC)、游离胆固醇(FC)含量,油红O染色结果观察细胞内脂质分布,以ELISA试剂盒检测炎性因子肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)的浓度。结果 CCK-8检测结果提示川芎嗪、芍药苷在80 mg/L浓度以下对泡沫细胞增殖活性没有明显影响;40 mg/L川芎嗪加载80 mg/L芍药苷可以明显降低泡沫细胞内TC、FC含量,减少泡沫细胞内脂质沉积,抑制泡沫细胞分泌TNF-α和IL-1β。结论芎芍胶囊有效成分(川芎嗪、芍药苷)能够协同降低RAW264.7源性泡沫细胞内胆固醇含量,并减少泡沫化细胞产生的炎症因子TNF-α、IL-1β,具备一定的抗炎作用。Aim To observe the effects of components of Xiongshao capsule on cholesterol and inflammatory factors on foam cells in vitro. Methods Oxidized low-density lipoprotein (ox-LDL) was used to induce the foaming of RAW264.7 cells. The proliferation activity of foam cells was detected by CCK-8 reagent. The total cholesterol (TC) and free cholesterol (FC) in foam cells were detected by cholesterol test kit, and the lipid distribution in cells was observed by oil red staining. The concentration of inflammatory factors, tumor necrosis factor alpha (TNF-α) and interleukin 1beta (IL-1β) was detected by ELISA kit. Results The results of CCK-8 showed that ligustrazine and paeoniflorin had no obvious effect on the proliferation of foam cells under 80 mg/L concentration. 40 mg/L ligustrazine plus 80 mg/L paeoniflorin could significantly reduce TC and FC contents in foam cells, reduce lipid deposition in foam cells, and inhibit the secretion of TNF-α and IL-1β by foam cells. Conclusion It is proved that the effective components of Xiongshao capsule, ligustrazine and paeoniflorin, can reduce the cholesterol content in foam cells derived from RAW264.7 cells and reduce the inflammatory factors, TNF-α and IL-1β produced by foam cells which have an potential ability to inhibit inflammatory reaction.

关 键 词：川芎嗪 芍药苷 泡沫细胞 炎症因子 

分 类 号：R5[医药卫生—内科学]