枳实芍药散对C-IBS大鼠VIP的调节作用  

Effects of Unripe Bitter Orange and Peony Powder on VIP Expression in Rats of Constipation-predominant Irritable Bowel Syndrome

作　　者：辛丹 季旭明[1] 张亚楠[1] 王媛[1] 王世军[1] 滕佳林[1] Xin Dan;Ji Xuming;Zhang Yanan(Shandong University of Traditional Chinese Medicine,Shandong 250355,China)

机构地区：[1]山东中医药大学

出　　处：《医学研究杂志》2019年第7期72-75,共4页Journal of Medical Research

基　　金：山东省自然科学基金资助项目(ZR2014HQ032);山东省重点产业关键技术项目(2016CYJS08A01-3,2016CYJS08A01-4);山东省重点研发计划项目(2016ZDJS07A12)

摘　　要：目的观察枳实芍药散对便秘型肠易激综合征(C-IBS)大鼠血管活性肠肽(VIP)表达的影响,探讨枳实芍药散治疗C-IBS的机制。方法将60只SPF级Wistar成年雄性大鼠随机分为空白组、模型对照组、匹维溴铵组、枳实芍药散高剂量组、枳实芍药散中剂量组和枳实芍药散低剂量组,每组10只,除了空白组,其余各组均采用冰水灌胃法建立C-IBS模型。模型制备成功后,空白组和模型对照组以26~28℃生理盐水按照10ml/(kg·d)的比例进行灌胃;匹维溴铵组以匹维溴铵混悬液0.0135g/(kg·d)治疗;枳实芍药散高剂量组、枳实芍药散中剂量组和枳实芍药散低剂量组各以枳实芍药散3.28g/(kg·d)、1.64g/(kg·d)、0.82g/(kg·d)治疗,各14天。计算并比较各组大鼠的小肠推进率,采用ELISA方法检测腹主动脉血清中VIP的表达。结果模型对照组大鼠的小肠推进率明显降低,匹维溴铵、高剂量组枳实芍药散、中剂量组枳实芍药散和低剂量组枳实芍药散治疗后小肠推进率明显增加。模型对照组大鼠的血清VIP的相对表达水平明显增高,匹维溴铵、高剂量组枳实芍药散、中剂量组枳实芍药散和低剂量组枳实芍药散治疗后血清VIP的相对表达水平明显下降。结论枳实芍药散可能通过调节血清VIP的表达从而改善C-IBS大鼠胃肠功能的紊乱。Objective To observe effects of Unripe bitter orange and Peony Powder on the expression of vasoactive intestinal peptide (VIP) in rats with constipation-predominant irritable bowel syndrome (C-IBS),and to explore the mechanism of Unripe bitter orange and Peony Powder in treating C-IBS.Methods Sixty adult male SPF Wistar rats were randomly divided into normal group,model control group,pinaverium bromide group,high dose of Unripe bitter orange and Peony Powder group,middle dose of Unripe bitter orange and Peony Powder group and low dose of Unripe bitter orange and Peony Powder group.There were 10 rats in each group.In addition to normal group,the other groups were established by ice water gavage method to establish C-IBS model.At the time of treatment,the high dose,middle dose and low dose of Unripe bitter orange and Peony Powder group ratswere given by gavage Unripe bitter orange and Peony Powder [3.28g/(kg·d),1.64g/(kg·d),0.82g/(kg·d)],the pinaverium bromide group rats were given by gavage with pinaverium bromide[0.0135g/(kg·d)],the blank group and the model control group rats were given by gavage once a day for 2 weeks with 26-28℃ saline[10ml/(kg·d)] simultaneously.After the treatment,the intestinal propulsion rate was calculated and compared,while the expression of VIP in abdominal aorta serum was detected by ELISA.Results The small intestinal propulsion rate of the model control group was significantly decreased.After treatment with pinaverium bromide and Unripe bitter orange and Peony Powder,the small intestinal propulsion rate was significantly increased.Compared with normal group,model control group,pinaverium bromide group,high dose of Unripe bitter orange and Peony Powder group,middle dose of Unripe bitter orange and Peony Powder group and low dose of Unripe bitter orange and Peony Powder group rats′ VIP expression levels obviously increased.Compared with model control group,the expression levels of VIP in high dose of Unripe bitter orange and Peony Powder group,middle dose of Unripe bitter orang

关 键 词：枳实芍药散 肠易激综合征 血管活性肠肽 

分 类 号：R2[医药卫生—中医学]