三物白散对Survivin-RNA基因沉默转染胃癌SGC-7901细胞凋亡、迁移的影响  

Effects of Sanwubai Powder on Apoptosis and Migration of Gastric Cancer SGC-7901 Cells Transfected with Survivin-RNA Gene Silencing

作　　者：范婧莹[1] 娄余 张娜 彭瑶[3] 朱莹 FAN Jingying;LOU Yu;ZHANG Na;PENG Yao;ZHU Ying(College of Integrated Chinese and Western Medicines, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China)

机构地区：[1]湖南中医药大学中西医结合学院,湖南长沙410208 [2]湖南中医药大学研究生院,湖南长沙410208 [3]湖南中医药大学第二附属医院,湖南长沙410005 [4]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2019年第8期937-942,共6页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助项目（81503561）;湖南省教育厅科研基金项目（16C1212）

摘　　要：目的探讨三物白散对Survivin-RNA基因沉默转染胃癌SGC-7901细胞凋亡、迁移的影响。方法将实验分成6组:空白对照组、LV-RNAi组、Survivin-RNAi-LV组、空白对照+中药组、LV-RNAi+中药组、Survivin-RNAi-LV+中药组;采用流式细胞仪检测各组细胞的凋亡,划痕实验检测各组细胞的迁移能力。结果细胞凋亡率:Survivin-RNAi-LV组明显高于空白对照组和LV-RNAi组(P<0.05);Survivin-RNAi-LV+中药组与空白对照+中药组、LV-RNAi+中药组比较明显较高(P<0.05);Survivin-RNAi-LV+中药组高于Survivin-RNAi-LV组,差异具有统计学意义(P<0.05)。胃癌细胞的24 h迁移率:Survivin-RNAi-LV组明显低于空白对照组和LV-RNAi组(P<0.05);Survivin-RNAi-LV+中药组与空白对照+中药组、LV-RNAi+中药组比较,其结果明显低于后两组(P<0.05);Survivin-RNAi-LV+中药组与Survivin-RNAi-LV组比较,细胞迁移率明显降低,差异有统计学意义(P<0.05)。结论Survivin基因沉默转染,增强了三物白散诱导胃癌SGC-7901细胞凋亡和抑制其迁移的作用。Objective To investigate the effects of Sanwubai Powder on apoptosis and migration of gastric cancer SGC-7901 cells transfected with Survivin-RNA gene silencing. Methods The experiment was divided into 6 groups:a blank control group, a LV-RNAi group, a Survivin-RNAi-LV group, a blank control group + TCM group, a LV-RNAi + TCM group, a Survivin-RNAi -LV + TCM group. The cell apoptosis was observed by flow cytometry, and the cell migration was assessed by cell scratch experiment. Results The apoptotic rate of the Survivin-RNAi -LV group was significantly higher than that of blank control group and LV-RNAi group (P<0.05);The apoptosis rate of the Survivin-RNAi-LV + TCM group was significantly higher than that of the blank control group + TCM group and the LV-RNAi + TCM group (P<0.05);The Survivin-RNAi-LV+ TCM group was higher than the Survivin-RNAi-LV group, and the difference was statistically significant (P<0.05). The 24 hour cell migration rate in the Survivin-RNAi -LV group was significantly lower than that in the blank control group and the LV-RNAi group (P<0.05). The Survivin-RNAi -LV + TCM group was significantly lower than that of the blank control group + TCM group and the LV-RNAi + TCM group (P<0.05);Compared with the Survivin-RNAi-LV group, the cell migration rate of the Survivin-RNAi-LV + TCM group was significantly decreased, and the difference was statistically significant (P<0.05). Conclusion After Survivin gene silencing transfection, the sensitivity of SGC-7901 cells to Sanwubai Powder was significantly enhanced, which further promoted the apoptosis of SGC-7901 cells and inhibited their migration.

关 键 词：SURVIVIN 三物白散 胃癌 细胞凋亡 迁移 

分 类 号：R285.5[医药卫生—中药学] R735.2[医药卫生—中医学]