多重PCR技术检测小儿咽拭子呼吸道病毒的临床分析  

Clinical Analysis of Multiplex PCR Technique for Detecting Respiratory Virus in Children with Throat Swabs

作　　者：樊旭成[1] 韩志国[1] 薛娜[1] 高枫[1] FAN Xu-cheng;HAN Zhi-guo;XUE Na;GAO Feng(Department of Infectious Disease Control,Urumqi Center for Disease Control and Prevention,Urumqi 830026,Xinjiang,China)

机构地区：[1]乌鲁木齐市疾病预防控制中心传染病防治科

出　　处：《医学信息》2019年第15期119-122,共4页Journal of Medical Information

基　　金：乌鲁木齐市科学技术计划重点实验室项目(编号:H171313001)

摘　　要：目的研究多重荧光聚合酶链式反应(PCR)技术对小儿咽拭子呼吸道病毒、病原菌检测的应用价值。方法筛选2018年1月~12月到乌鲁木齐市社区服务门诊就诊的社区获得性肺炎(CAP)患儿524例,采用多重PCR技术检测患儿咽拭子病原菌,分析CAP病原菌的分布情况及其与年龄、季节等的关系。结果CAP病原检测结果大部分属于病毒性,以乙型流感病毒、肺炎衣原体、肺炎支原体等为主,细菌类以肺炎克雷伯菌等为主;与患儿年龄、季节有关,3岁以下患儿仍是CAP的主要发病年龄段,尤其是婴幼儿最多,发病多集中在秋季和冬季;2种以上病原混合感染形式严峻。结论多重PCR技术对小儿咽拭子病原菌检测的应用效果良好,快捷方便。Objective To study the application value of multiplex fluorescent polymerase chain reaction (PCR) in the detection of respiratory viruses and pathogens in children with throat swabs.Methods A total of 524 children with community-acquired pneumonia (CAP) who were treated in the community service clinic in Urumqi from January to December 2018 were enrolled. The phage swab pathogens were detected by multiplex PCR and the distribution of CAP pathogens was analyzed. Relationship between age, season, etc.Results Most of the results of CAP pathogen detection were viral, mainly influenza B virus, Chlamydia pneumoniae, Mycoplasma pneumoniae, and bacteria such as Klebsiella pneumoniae;related to age and season of children, under 3 years old children are still the main age of onset of CAP, especially in infants and young children. The incidence is mostly concentrated in autumn and winter;the mixed forms of more than two pathogens are severe.Conclusion The multiplex PCR technique has a good effect on the detection of pathogenic bacteria in children's throat swabs, which is quick and convenient.

关 键 词：多重PCR技术 小儿咽拭子 呼吸道病毒 病原菌 

分 类 号：R373.1[医药卫生—病原生物学]