精胺对雌鹅十二指肠多胺代谢关键基因表达及多胺含量的影响  

Effect of Spermine on Expression of Key Genes Related to Polyamine Metabolism and Polyamine Contents in the Duodenum of Female Geese (Anser cygnoides)

作　　者：杜菲 易治鑫 于海莹 张燕[1] 李慧敏 杨莉 王钰曾 姜冬梅[1] 康波[1] DU Fei;YI Zhi-Xin;YU Hai-Ying;ZHANG Yan;LI Hui-Min;YANG Li;WANG Yu-Zeng;JIANG Dong-Mei;KANG Bo(College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China)

机构地区：[1]四川农业大学动物科技学院

出　　处：《农业生物技术学报》2019年第8期1445-1451,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.31872358和No.31201798);四川省教育厅青年基金(No.18ZB0469)

摘　　要：精胺是细胞增殖和分化的必需因子之一,不仅参与调节肠道上皮细胞增殖与凋亡、促进肠道发育,还参与调控肠道消化与吸收功能。为研究外源性精胺对四川白鹅(Anser cygnoides)雌鹅十二指肠多胺代谢的影响,按照鹅体重灌胃5和10 mg/kg精胺,应用qRT-PCR和高效液相色谱技术检测雌鹅十二指肠多胺代谢关键基因表达量及多胺含量。结果表明,5 mg/kg精胺灌胃组雌鹅十二指肠组织鸟氨酸脱羧酶抗酶抑制剂(ornithine decarboxylase antizyme inhibitor, AZIN) 1、鸟氨酸脱羧酶抗酶1 (ornithine decarboxylase antizyme 1, OAZ1)、精胺合成酶(spermine synthase, SPMS)、亚精胺/精胺N1-乙酰转移酶(spermidine/spermine-N1-acetyltransferase, SSAT)和乙酰多胺氧化酶(acetyl-polyamine oxidase, APAO)基因表达量均显著高于对照组(P<0.05);AZIN2基因表达量显著低于对照组(P<0.05);10 mg/kg精胺灌胃组雌鹅十二指肠AZIN1、OAZ1、鸟氨酸脱羧酶(ornithine decarboxylase, ODC)、亚精胺合成酶(spermidine synthase, SPDS)和SSAT的基因表达量均显著高于对照组(P<0.05)。5 mg/kg精胺灌胃组雌鹅十二指肠亚精胺(65.08 vs 41.84 mg/kg)和精胺(255.79 vs 218.84 mg/kg)含量显著高于对照组(P<0.05);10 mg/kg精胺灌胃组雌鹅十二指肠亚精胺(56.20 vs 41.84 mg/kg)含量显著高于对照组(P<0.05);而精胺(188.34 vs218.84 mg/kg)含量显著低于对照组(P<0.05)。综上所述,外源性精胺灌胃可通过介导多胺代谢关键酶基因表达而影响雌鹅十二指肠组织中亚精胺和精胺含量,对腐胺含量无显著影响。本研究揭示了外源性精胺对雌鹅十二指肠多胺代谢关键基因表达及多胺含量的影响,为精胺调控家禽消化与吸收的相关研究提供了基础数据,并可为开发新型功能性饲料提供新途径。Spermine is one of the essential factors for cell proliferation and differentiation. The spermine in small intestine not only plays a role in regulating intestinal epithelial cell proliferation and apoptosis and promoting intestinal development, but also plays a role in regulating intestinal digestion and absorption. This experiment was conducted to study the effects of exogenous spermine on polyamine metabolism in female goose(Anser cygnoides) duodenum. Geese were fed spermine at 5 and 10 mg/kg(body weight). The expression of key genes related to polyamine metabolism and the content of polyamines in female geese duodenum were measured by qRT-PCR and high performance liquid chromatography. The gene expression levels of ornithine decarboxylase antizyme inhibitor(AZIN) 1, ornithine decarboxylase antizyme 1(OAZ1), spermine synthase(SPMS), spermidine/spermine-N1-acetyltransferase(SSAT) and acetyl-polyamine oxidase(APAO) in the duodenum of female geese treated with spermine at 5 mg/kg were significantly higher than those in the control group(P<0.05), while the gene expression level of AZIN2 significantly decreased(P<0.05). The gene expression levels of AZIN1, OAZ1, ornithine decarboxylase(ODC), spermidine synthase(SPDS) and SSAT in the duodenum of female geese treated with spermine at 10 mg/kg were significantly higher than those in the control group(P<0.05). The contents of spermidine(65.08 vs 41.84 mg/kg) and spermine(255.79 vs 218.84 mg/kg) in the duodenum of female geese treated with spermine at 5 mg/kg were significantly higher than those in the control group(P<0.05). Spermidine content(56.20 vs 41.84 mg/kg) in the duodenum of female geese treated with spermine at 10 mg/kg was significantly higher than that in the control group(P<0.05), while spermine content(188.34 vs 218.84 mg/kg) significantly decreased(P<0.05). In summary, exogenous spermine affects the contents of spermidine and spermine in female goose duodenum through regulating the expression of key genes related to polyamine metabolism, while it had no s

关 键 词：四川白鹅 精胺 十二指肠 多胺代谢 基因表达 

分 类 号：S835[农业科学—畜牧学]