液相流路控制与微流控核酸扩增芯片偶联系统的研制  

Development of Microfluidic Nucleic Acid Amplification Chip Coupled with Liqid Flow Control System

作　　者：刘可夫[1] 郑福 张泽宇 翟光杰 庆宏[1] Kefu Liu;Fu Zheng;Zeyu Zhang;Guangjie Zhai;Hong Qing(School of Life Science, Beijing Institute of Technology, Beijing 100081;National Space Science Center, Chinese Academy of Sciences, Beijing 100190;School of Chemical Engineering and Technology,North University of China, Taiyuan 030051)

机构地区：[1]北京理工大学,北京100081 [2]中国科学院国家空间科学中心,北京100190 [3]中北大学,太原030051

出　　处：《生命科学仪器》2019年第3期53-57,共5页Life Science Instruments

基　　金：国家重大科学仪器设备开发专项课题(2012YQ04014004)

摘　　要：随着载人航天和空间探测技术的进步,空间站特殊环境导致常规仪器无法直接在空间站内正常运行,使得改进和开发适用于空间站的生物分析检测设备变得非常重要。其中核酸作为一个重要的生物大分子,在生物科学研究中对它的分析是必不可少的。本研究针对核酸扩增设备在航天中的应用提出了一种液相流路偶联微流控核酸扩增芯片系统来解决微重力环境下核酸检测过程中对于液体处理的问题,实验结果表明液体混合良好,密封性良好,能满足微重力条件的液体传输。这一系统为基于微流控芯片的空间核酸分析设备的开发提供思路。With the development of space technology and the inapplicability of conventional devices in microgravity environment, it is more and more important to improve or develop biological analysis and detection device apply to the space station. Nucleic acid, as a most important biomacromolecule, is necessary to analyze in biology researches. This study put forward a kind of microfluidic chip for nucleic acid amplification coupling liquid flow control system to solve liquid handling problem in the microgravity environment for nucleic acid detection. The results show that the system is good sealing and buffer mixture sufficient. It can achieve liquid transfer in the microgravity conditions. It can provide new idea in development of space nucleic acid analysis devices in space.

关 键 词：微流控芯片 液相流路控制 核酸扩增 空间站 

分 类 号：Q3[生物学—遗传学]