利用KASP分子标记技术辅助筛选多抗番茄材料  

Auxiliary Screening of Polymeric Multi-resistance Tomato Materials Using KASP Molecular Marker Technology

作　　者：李宗俊 王先裕[1] 刘梦姣 崔馨月 赵雄 陈鹏[1] 欧青青 LI Zong-jun;WANG Xian-yu;LIU Meng-jiao;CUI Xin-yue;ZHAO Xiong;CHEN Peng;OU Qingqing(College of Agriculture,Guangxi University,Nanning 530001,Guangxi,China)

机构地区：[1]广西大学农学院

出　　处：《中国蔬菜》2019年第8期42-46,共5页China Vegetables

基　　金：国家大宗蔬菜产业技术体系试验站项目(CARS-25-G-37);国家重点研发计划项目(2017YFD0101902-5);大学生创新创业训练计划项目(201810593284);广西重点研发计划项目(2017AB50047)

摘　　要：广西大部分地区春秋两季阴雨天气较多、降雨量偏高,利于晚疫病产生的致病疫霉(Phytophthora infestans)及其孢子囊的存活及传播。利用田间抗性鉴定与 KASP 分子标记技术,对 16 份番茄材料进行晚疫病抗性检测,筛选聚合多抗材料。结果表明:10 份材料田间对晚疫病表现高抗或抗病;3 份材料含晚疫病纯合体抗性基因 Ph-3,2 份材料含番茄黄化曲叶病毒病纯合体抗性基因 Ty-1,4 份材料含番茄黄化曲叶病毒病纯合体抗性基因 Ty-3,10 份材料含黄萎病纯合体抗性基因 Ve-2,LF-15、LF-35、LF-41 等 3 份材料同时含有 4 种抗性基因,其中 LF-35 含有的 4 种抗性基因皆为纯合体。In most areas of Guangxi,there are more rainy days in spring and autumn and the rainfall is higher.This climatic conditions are bene ficial to the survival and pathophoresis of Phytophthora infestans and sporangium produced by late blight.This paper conducted resistance test for late blight on 16 large-fruit tomato materials to screen Polymeric multi-resistant materials using field resistance identification and KASP molecular marker technology.The results showed that 10 materials showed high resistant or resistant to late blight in fields;3 materials contained late blight homozygous resistance gene Ph-3;2 materials contained TYLCV homozygous resistance gene Ty-1;4 materials contained TYLCV homozygous resistance gene Ty-3;10 materials contained Verticillium wilt homozygous resistance gene Ve-2.Besides,3 materials:LF-15,LF- 35,LF-41 had 4 resistant genes at the same time.Among them,LF-35 contained 4 resistant genes all being homozygote.

关 键 词：KASP 分子标记 番茄 聚合多抗 抗性鉴定 

分 类 号：S641.2[农业科学—蔬菜学]