基于水稻线粒体atp6基因的稻属CMS基因起源及DNA条形码的研究  

Study on the Origin of CMS Gene and DNA Barcode for Oryza Based on Rice Mitochondrial atp6 Gene

作　　者：李平 刘雅婷 赵自仙[1] 娄红波[1] 张雪梅[1] LI Ping;LIU Yating;ZHAO Zixian;LOU Hongbo;ZHANG Xuemei(College of Agriculture and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;Academic Affairs Office,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学教务处,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2019年第4期555-563,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金(31200908)

摘　　要：【目的】探讨亚洲栽培稻CMS基因起源及水稻线粒体atp6特异性引物作为稻属DNA条形码标记的应用。【方法】用水稻atp6特异性引物通过PCR检测产于中国的4种水稻,共720个个体,并对111个个体测序。【结果】普通野生稻和亚洲栽培稻中均检测到atp6保守序列。药用野生稻中没检测到扩增产物。疣粒野生稻中检测到的5个单倍型间共计17个变异位点。澜沧、普洱、勐海居群共同拥有H1,单倍型H2、H3、H4和H5分别为新平、墨江、保亭和崖城居群特有。云南组和海南组,组内平均遗传距离为0,组间为0.02;组间遗传分化系数Fst为92.08%;变异位点数(S)、单倍型数(h)、核苷酸多样性(Pi)均显示:云南组(4、3、0.00201)遗传多样性大于海南组(1、2、0.00131);云南组单倍型多样性Hd为0.6,海南组为1。5个单倍型与水稻atp6保守序列的相似度最高为45.19%。最大似然法ML进化树显示:澜沧、普洱及勐海一带可能是疣粒野生稻的起源地,海南疣粒野生稻可能源自大陆。【结论】水稻atp6特异性引物可作为疣粒野生稻种内条形码标记,该标记也可区分普通野生稻、药用野生稻和疣粒野生稻。从线粒体DNA角度证实亚洲栽培稻CMS基因源自普通野生稻。[Purpose]To explore the origin of CMS gene in Oryza sativa and the application of the rice mitochondrial rice atp6 specific primer as Oryza DNA barcode markers.[Method]The atp6 specific primer were used to detect 4 kinds of rice produced in China by PCR, a total of 720 individuals, and 111 individuals were sequenced.[Result]Conserved sequence of atp6 was detected in O. rufipogon and O. sativa. No amplification products were detected in O. officinalis. A total of 17 variation locus between the five haplotypes were detected in O. meyeriana. Lancang, Pu'er, Menghai population shared H1. Haplotype H2, H3, H4 and H5 were unique to Xinping, Mojiang, Baoting and Yacheng population respectively.Yunnan group and Hainan group, average genetic distance within the group were all 0, and between groups was 0.02. The genetic differentiation coefficient Fst of between groups was 92.08%. Number of variant sites (S), number of haplotypes (h) and nucleotide diversity (Pi) showed that the genetic diversity of Yunnan group (4, 3, 0.002 01) was greater than that in Hainan group (1, 2, 0.001 31). The haplotype diversity Hd of the Yunnan group was 0.6, and that in Hainan was 1. The similarity between 5 haplotypes and the rice atp6 conserved sequence was up to 45.19%. Maximum likelihood (ML) evolutionary tree displayed: the Lancang, Pu ’er, Menghai area might be the origin of the O. meyeriana and Hainan O. meyeriana might originate from the mainland.[Conclusion]Rice atp6 specific primers can be used as barcode markers within O. meyeriana species. The marker can also distinguish between O. rufipogon, O. officinalis, and O. meyeriana. O. sativa CMS gene is derived from O. rufipogon from the perspective of mitochondrial DNA.

关 键 词：atp6基因 水稻 序列分析 起源 DNA条形码 

分 类 号：S511.032[农业科学—作物学]