复配拮抗菌FP246对烟草黑胫病和根际土壤微生物群落功能多样性的影响  

Effects of combinatory antagonistic bacteria FP246 on tobacco black shank and functional diversity of rhizosphere soil microbial communities

作　　者：张维 刁朝强 李斌 包自超 张家韬 林北森 陈德鑫 ZHANG Wei;DIAO Chaoqiang;LI Bin;BAO Zichao;ZHANG Jiatao;LIN Beisen;CHEN Dexin(Institute of Tobacco Research of CAAS,Qingdao 266100,Shandong,China;Guiyang Branch of Guizhou Provincial Tobacco Corporation,Guiyang 550001,China;Sichuan Provincial Tobacco Corporation of CNTC,Chengdu 610041,China;Shandong Provincial Tobacco Corporation of CNTC,Jinan 250101,China;Shaanxi Provincial Tobacco Corporation of CNTC,Xi’an 710000,China;Hainan Provincial Tobacco Corporation of CNTC,Haikou 571199,China)

机构地区：[1]中国农业科学院烟草研究所,山东省青岛市266100 [2]贵州省烟草公司贵阳市公司,贵阳市550001 [3]中国烟草总公司四川省公司,成都市610041 [4]中国烟草总公司山东省公司,济南市250101 [5]中国烟草总公司陕西省公司,西安市710000 [6]中国烟草总公司海南省公司,海口市571199

出　　处：《烟草科技》2019年第7期10-17,共8页Tobacco Science & Technology

基　　金：贵州省烟草专卖局(公司)重点科技项目“贵阳烟草主要病虫草绿色防控技术集成示范与推广”(201606);四川省烟草专卖局(公司)重点科技项目“四川烟草主要病害绿色防控技术集成示范与推广”(SCYC201604);山东省烟草专卖局(公司)重点科技项目“山东烟草主要病虫草绿色防控技术集成示范与推广”(KN238)

摘　　要：为研究复配拮抗菌FP246对烟草黑胫病的防治效果及其对根际土壤微生物群落功能的影响,通过盆栽试验研究其对烟草黑胫病的防效,并利用Biolog-ECO技术分析施入复配拮抗菌FP246后根际土壤微生物群落功能多样性的变化。结果表明,复配拮抗菌FP246能显著降低黑胫病的发病程度,防效为85.40%。Shannon-Wiener多样性指数结果显示,复配拮抗菌FP246处理(T1处理)与58%甲霜锰锌可湿性粉剂处理(T3处理)无显著差异,但与10亿个/g枯草芽孢杆菌可湿性粉剂处理(T2处理)及对照(T4处理)差异显著;McIntosh指数及丰富度指数大小整体为T3>T1>T2>T4;T1、T2、T3处理的Simpson优势度指数没有明显变化。T1、T2、T3处理的AWCD值均显著增加,且与对照差异显著。主成分分析发现,微生物在96~144h对碳源的利用较为活跃,其中对糖类、氨基酸、羧酸类以及聚合物类碳源利用较多。To investigate the control efficiency of combinatory antagonistic bacteria FP246 against tobacco black shank and its influence on the functional variations of soil microbial community, a pot experiment was conducted and the functional diversity of rhizosphere soil microbial community was analyzed by Biolog-ECO after FP246 treatment. The results showed that the incidence of tobacco black shank was reduced by FP246 treatment with the control efficiency of 85.40%. No significant difference was found in Shannon-Wiener diversity index between FP246 treatment (T1) and that treated by a 58% Fubol wettable powder (T3), while the differences from 109 spore/g Bacillus subtilis wettable powder treatment (T2) and the control (T4) were statistically significant. The McIntosh index rank and the richness index was in the order of T3>T1>T2>T4. Simpson’s dominance indexes did not significantly differ among T1, T2 and T3. Comparing with the control, the AWCD values in T1, T2 and T3 were significantly increased and significantly differed from that of the control. A principal component analysis indicated that carbon source utilization of microorganisms was higher from 96 to 144 h, especially sugars, amino acids, carboxylic acids and polymers.

关 键 词：烟草黑胫病 生防菌剂 土壤微生物 Biolog-ECO 多样性 

分 类 号：S43[农业科学—农业昆虫与害虫防治] TS414[农业科学—植物保护]