271例儿童支气管哮喘过敏原特异性IgE抗体检测分析  

Analysis of serum allergen-specific IgE in 271 children with bronchial asthma

作　　者：牛超 宋晓妍[2] 杜红梅[2] 李付广[1] NIU Chao;SONG Xiao-yan;DU Hong-mei;Li Fu-guang(Department of Immunology,Zhengzhou University School of Basic Medicine,Zhengzhou 450001 China;Department of Clinical laboratory,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学基础医学院免疫教研室,河南郑州450001 [2]郑州大学第三附属医院检验科,河南郑州450052

出　　处：《河南医学研究》2019年第14期2519-2521,共3页Henan Medical Research

摘　　要：目的探讨0~12岁支气管哮喘患儿的哮喘发病与其血清中特异性IgE抗体表达的关系及其检测意义。方法采用欧蒙印迹法对2015年6月至2018年6月就诊于郑州大学第三附属医院的271例支气管哮喘患儿进行血清过敏原特异性lgE抗体检测,并对结果进行回顾性分析。结果 271例支气管哮喘患儿中抗体阳性患儿203例,阳性率74.91%,其中吸入性过敏原阳性率较高的是蟑螂(31.03%)、尘螨组合(25.62%);食物性过敏原阳性率较高的是花生(25.62%)、鸡蛋白(20.69%)、海鱼组合(10.83%)。男性患儿中过敏原阳性者129例,阳性率为85.43%,女性患儿中过敏原阳性者74例,阳性率为61.67%。男性患儿过敏原阳性率高于女性患儿,差异有统计学意义(P<0.05)。过敏原阳性率的年龄分布情况为0~4岁(82.61%),5~8岁(75.00%),9~12岁(62.50%)。三年龄段间比较,差异有统计学意义(P<0.05)。0~4岁患儿过敏原抗体阳性率与5~8岁患儿比较,差异无统计学意义(P=0.190);0~4岁患儿过敏原抗体阳性率高于9~12岁患儿,差异有统计学意义(P=0.002);5~8岁患儿过敏原抗体阳性率与9~12岁患儿比较,差异无统计学意义(P=0.092)。结论蟑螂、尘螨组合、花生、鸡蛋白为哮喘患儿最常见过敏原,且吸入性过敏原与哮喘的发生关系更为密切。Objective To investigate the relationship between the incidence of asthma and specific IgE antibody expression in serum of children with bronchial asthma aged 0-12 years and its significance in detection. Methods A total of 271 cases of children with bronchial asthma admitted to the third affiliated hospital of zhengzhou university from June 2015 to June 2018 were tested by EUROBlot with serum allergene-specific lgE antibody,and the results were retrospectively analyzed. Results Among the 271 children with bronchial asthma,203 cases were antibody positive,with a positive rate of 74.91%. Among them,cockroach 31.03% and dust mite 25.62% had the highest positive rate of inhalation allergen. The positive rate of food allergen was 25.62% for peanut,20.69% for egg white and 10.83% for marine fish. Among the male children,129 cases had positive allergen,with a positive rate of 85.43%. Among the female children,74 cases had positive allergen,with a positive rate of 61.67%. The positive rate of allergen in male children was significantly higher than that in female children ( P < 0.05). The age distribution of allergen positive rate was 0-4 years old (82.61%),5-8 years old (75.00%) and 9-12 years old (62.50%). There was significant difference in the positive rate of allergen antibody among the three age groups ( P < 0.05). There was no significant difference in the positive rate of allergen antibody between the children aged 0-4 years and 5-8 years old ( P = 0.190). The positive rate of allergen antibody in children aged 0-4 years was higher than that in children aged 9-12 years old,the difference was statistically significant ( P = 0.002). There was no significant difference in the positive rate of allergen antibody in children aged 5-8 years and 9-12 years( P = 0.092). Conclusion Cockroach,dust mite combination,peanut and egg white are the most common allergens in children with asthma,and inhaled allergens are more closely related to the occurrence of asthma.

关 键 词：支气管哮喘 过敏原 儿童 特异性IGE 

分 类 号：R725[医药卫生—儿科]