新疆石河子地区绵羊源胰阔盘吸虫的分离及分子鉴定  

Isolation of Sheep-origin Eurytrema Pancreaticum in Shihezi Region and Its Molecular Identification

作　　者：王正荣[1,2] 张艳艳 徐雪萍[1,2] 杨华 薄新文[1,2] WANG Zheng-rong;ZHANG Yan-yan;XU Xue-ping;YANG Hua;Bo Xin-wen(Institute of Animal Husbandry and Veterinary Medicine,Xinjiang Academy of Agricultural Reclamation,Shihezi 832000,China;State Key Laboratory of Sheep Genetic Improvement and Healthy Production,Shihezi 832000,China)

机构地区：[1]新疆农垦科学院畜牧兽医研究所,新疆石河子832000 [2]省部共建绵羊遗传改良与健康养殖国家重点实验室,新疆石河子832000

出　　处：《草食家畜》2019年第4期30-34,共5页Grass-Feeding Livestock

基　　金：新疆生产建设兵团重大科技项目(2017AA006)

摘　　要：本研究通过对胰阔盘吸虫18SrRNA部分基因序列的分析,研究了新疆石河子地区绵羊胰阔盘吸虫的感染情况。试验检查了47只绵羊的胰腺吸虫感染情况,根据其典型形态特征进行分类。同时利用PCR技术扩增其部分18SrRNA基因序列,并做进化分析。研究结果表明:1)被检的47只羊中6只检出胰阔盘吸虫,感染率为12.77%;2)PCR的鉴定结果与形态学鉴定结果相吻合;3)在石河子地区应注重绵羊胰阔盘吸虫病的防控。In this study, the 18S rRNA gene sequence of E.pancreaticum was analyzed and the infection of E. pancreaticum of sheep in Shihezi region was investigated. 47 sheep were detected for E. pancreaticum that was classified as per its typical morphological characteristics. At the same time, some 18S rRNA gene sequences were amplified by polymerase chain reaction (PCR) and analyzed for evolution. The results showed that 6 of the 47 sheep were positive for E. pancreaticum with the infection rate of 12.77%, and the results of PCR were consistent with that of morphological identification. Thus,it was suggested that the prevention and control of eurytrematosis should be strengthened in Shihezi region.

关 键 词：绵羊 胰阔盘吸虫 分子鉴定 

分 类 号：S54[农业科学—作物学]