基于SNP标记的玉米雄穗主要性状QTL定位分析  

QTL Analysis of Tassel Traits Based on SNP Markers in Maize

作　　者：贾波[1] 崔敏 谢庆春[1,3] 严卫古 印志同[2] JIA Bo;CUI Min;XIE Qing-chun;YAN Wei-gu;YIN Zhi-tong(Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Jiangsu Huaian 223001, China;Agricultural College of Yangzhou University, Jiangsu Yangzhou 225009, China;Jiangsu Collaborative Innovation Center of Regional Modem Agriculture & Environmental Protection, Jiangsu Huaian 223300, China)

机构地区：[1]江苏徐淮地区淮阴农业科学研究所,江苏淮安223001 [2]扬州大学农学院,江苏扬州225009 [3]江苏省区域现代农业与环境保护协同创新中心,江苏淮安223300

出　　处：《西南农业学报》2019年第7期1469-1473,共5页Southwest China Journal of Agricultural Sciences

基　　金：江苏省重点研发计划(BE2017365-3);扬州大学中青年学术带头人项目;江苏省前瞻性联合研究(产学研)项目(BY2016069-09);江苏省环洪泽湖生态农业生物技术重点实验室自主研发课题(17HZHL008);淮安市农业科学研究院院长科研基金项目(HNY201710)

摘　　要：【目的】雄穗性状是玉米生长发育过程中重要的农艺性状,对其遗传特性的研究具有重要的理论意义。【方法】本研究以Z58×Y915构建的192个F_(2:3)家系作为作图群体,结合2个环境下的表型鉴定,运用复合区间作图法(CIM)对玉米雄穗长(TL)、雄穗分枝数(TBN)和雄穗重(TW)等雄穗性状进行QTL定位分析。【结果】2个环境条件下,共检测到15个与TL性状连锁的QTL位点,分别位于第1、3、4、5、6、7、8号染色体上,可解释表型变异的0.66%~22.58%;在染色体bin值1.09、3.09位置,2个环境中均稳定检测到与TL连锁的QTL位点;共检测到12个与TBN性状连锁的QTL位点,分别位于第1、2、3、5、6、7、9号染色体上,可解释表型变异的3.05%~23.80%;在染色体bin值2.08、3.09位置,2个环境中均稳定检测到与TBN连锁的QTL位点;共检测到9个与TW性状连锁的QTL位点,分别位于第1、3、4、6、7、9号染色体上,可解释表型变异的4.52%~27.55%,在染色体bin值3.09、9.05位置,2个环境中均稳定检测到与TW连锁的位点。【结论】在不同环境下能够稳定存在的QTL位点可为玉米雄穗主要性状进一步遗传研究提供理论基础。【Objective】The tassel traits were very important in the growth and development of maize, the research of their genetic characteristics was of great significance.【Method】In this study, 192 F2:3 family lines derived from Z58 ×Y915 were used to identify QTL for the tassel length(TL),the tassel branch number(TBN) and the tassel weight(TW) with composite interval mapping(CIM) in two different environments. 【Result】15 QTL for TL were mapped to chromosomes 1, 3, 4, 5, 6, 7 and 8, and these QTL could explain the phenotypic variation ranging from 0.66 % to 22.58 % in two environments. The QTL in bin 1.09 and bin 3.09 were stably detected across two environments. 12 QTL for TBN were detected in two environments, which were respectively at 1, 2, 3, 5, 6, 7 and 9 chromosomes, and these QTL could explain the phenotypic variation ranging from 3.05 % to 23.80 % in two environments. The QTL in bin 2.08 and bin 3.09 were stably detected across the different environments. 9 QTL for TW were mapped to chromosomes 1, 3, 4, 6, 7 and 9, these QTL could explain the phenotypic variation ranging from 4.52 % to 27.55 % in the different environments, and the QTL in bin 3.09 and bin 9.05 were stably detected across the different environments. 【Conclusion】These QTL that were stably detected across the different environments could provide a valuable reference for genetic research in maize.

关 键 词：玉米 雄穗长 雄穗分枝数 雄穗重 

分 类 号：S513[农业科学—作物学]