玉郎伞查尔酮对大鼠心肌缺血再灌注损伤后炎性反应的影响  

Effects of 17-methoxyl-7-hydroxyl-benzofuran chalcone on inflammatory responses in myocardial ischemia reperfusion injury in rats

作　　者：覃斐章[1] 秦秋华 QIN Feizhang;QIN Qiuhua(Department of Pharmacology,College of Pharmacy,Guangxi Medical University,Guangxi Zhuang Autonomous Region,Nanning 530021,China;Department of Pharmacy,Nanning Second People's Hospital,Guangxi Zhuang Autonomous Region,Nanning 530031,China)

机构地区：[1]广西医科大学药学院药理教研室,广西南宁530021 [2]南宁市第二人民医院药剂科,广西南宁530031

出　　处：《中国医药导报》2019年第20期13-16,33,共5页China Medical Herald

基　　金：广西自然科学基金面上项目(2017GXNSFAA198145)

摘　　要：目的观察玉郎伞查尔酮(YLSC)对大鼠心肌缺血再灌注后炎性反应的影响。方法将60只12周龄的SPF级SD大鼠按随机数字表法分为假手术组(等体积生理盐水),模型组(等体积生理盐水),阳性组(葛根素注射液12.00mg/kg),YLSC低剂量组(2.50mg/kg)、中剂量组(YLSC5.00mg/kg)和高剂量组(YLSC10.00mg/kg),每组10只,雌雄各半。缺血1h再灌注3h复制大鼠缺血再灌注模型。假手术组只穿线不结扎。采用酶联免疫吸附法检测大鼠血清MB型肌酸激酶(CK-MB)、肌钙蛋白(cTnI)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的含量。伊文思蓝和2,3,5-三苯基氯化四氮唑双重染色确定心肌梗死面积。Westernblot法检测心肌高迁移率族蛋白1(HMGB1)、Toll样受体4(TLR4)和核转录因子κB(NF-κB)。结果与模型组比较,YLSC能减少心肌酶CK-MB、cTnI的漏出(P<0.05或P<0.01),缩小心肌梗死面积(P<0.05或P<0.01),降低血清炎症因子IL-6、IL-1β、TNF-α的释放(P<0.05或P<0.01),并抑制心肌HMGB1、TLR4、NF-κB的蛋白表达(P<0.05或P<0.01)。结论YLSC能减少大鼠缺血再灌注损伤,其心肌保护作用可能与减轻炎性反应,抑制心肌HMGB1、TLR4、NF-κB的蛋白表达有关。Objective To observe the effects of 17-methoxyl-7-hydroxyl-benzofuran chalcone (YLSC) on inflammatory responses in myocardial ischemia reperfusion injury in rats.Methods Sixty 12-month-old SPF Sprague-Dawley (SD) rats were randomly divided into sham group (equal volume of normal saline),model group (equal volume of normal saline),positive control group (Puerarin Injection,12.00 mg/kg) and YLSC low,medium and high-dose groups (2.50,5.00,10.00 mg/kg) via random number table method.The anterior descending coronary artery was ligated for 1 h then reperfused for 3 h to establish the ischemia reperfusion (I/R) model.Rats in sham group were applied stitches under the coronary artery without ligation.Levels of serum troponin I (cTnI),creatine kinase-MB (CK-MB),interleukin-6 (IL-6),interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) were examined by enzyme-linked immunosorbent assay.The infarct sizes were measured by evans blue and 2,3,5-triphenyltetrazolium chloride staining.Protein levels of high mobility group box-1(HMGB1),toll-like receptors 4 (TLR4) and nuclear factor-kappa B (NF-κB) in myocardiums were evaluated using Western blot.Results Compared with model group,YLSC reduced the leakage of myocardial enzyme CK-MB and cTnI (P < 0.05 or P < 0.01),decreased myocardial infarction size (P < 0.05 or P < 0.01),lower the release of serum inflammatory cytokines such as IL-6,IL-1β and TNF-α(P < 0.05 or P < 0.01),and inhibited the protein expression of myocardial HMGB1,TLR4,NF-κB (P < 0.05 or P < 0.01).Conclusion YLSC can reduce ischemia-reperfusion injury in rats,the myocardial protective effect may be related to reducing of inflammatory reaction,inhibiting the expression of myocardial related protein such as HMGB1,TLR4 and NF-κB.

关 键 词：玉郎伞查尔酮 心肌缺血再灌注 高迁移率族蛋白1 炎性反应 

分 类 号：R542.22[医药卫生—心血管疾病]