浙江省甘薯上发现瓜类细菌性果斑病菌  

Pathogen identification of bacterial fruit blotch of melon on sweet potato in Zhejiang Province

作　　者：洪纤纤 吴秀芹 罗金燕[2] 王艳丽[3] 孙国仓 安千里[1] 邱文[1] 李斌[1] HONG Xianxian;WU Xiuqin;LUO Jinyan;WANG Yanli;SUN Guocang;AN Qianli;QIU Wen;LI Bin(Key Laboratory of Molecular Biology of Crop Pathogens and Insects,Ministry of Agriculture and Rural Affairs,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China;Shanghai Agriculture Technology Extension & Service Center,Shanghai 201103,China;Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China.)

机构地区：[1]浙江大学生物技术研究所,农业农村部作物病虫分子生物学重点实验室,浙江杭州310058 [2]上海市农业技术推广服务中心,上海201103 [3]浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《浙江农业学报》2019年第7期1112-1118,共7页Acta Agriculturae Zhejiangensis

基　　金：浙江省重点研发项目(2017C02002);浙江省自然科学基金(LZ19C140002,LY19C010007);上海市农业基础性研究项目(沪农科攻字[2014]第7-3-1号,沪农科攻字[2015]第4-4号)

摘　　要：通过对浙江宁海县疑似甘薯茎腐病等检疫性病害的调查,在甘薯病株上分离纯化获得能够引起烟草过敏反应的4株分离物,通过菌落形态观察、革兰氏染色反应、16S rRNA序列分析以及致病性测定等细菌学测定,将这4株菌株都鉴定为西瓜噬酸菌( Acidovorax citrulli ,原 Acidovorax avenae subsp . citrulli ),序列同源性高达99.00%~100.00%。瓜类细菌性果斑病菌为我国的检疫性有害生物,务必引起高度重视,以保障浙江重要经济作物西甜瓜产业的健康发展。Bacterial stem and root rot of sweet potato is an important quarantine disease. In the current study, four isolates of suspected diseased sweet potato induced a hypersensitive response on tobacco plants. These isolates were then identified into Acidovorax citrulli (formerly Acidovorax avenae subsp . citrulli ) based on morphology, Gram s stain reaction, 16S rRNA sequence and pathogenicity analysis. Results here showed that the sequence homology of the novel isolates was as high as 99.00%-100.00% compared with A. citrulli, the bacterial pathogen of bacterial fruit blotch of cucurbits, which was the key quarantine pathogen of China. The current report indicated the importance of disease management for efficient controlling of bacterial fruit blotch of watermelon in Zhejiang Province.

关 键 词：甘薯 西瓜噬酸菌 西瓜果斑病 鉴定 检疫 

分 类 号：S435.311[农业科学—农业昆虫与害虫防治]