冠心病并发幽门螺杆菌感染患者血清HP抗体谱特征与炎性因子水平相关性分析  

Correlation Analysis of Serum Antibody Spectrum and Inflammatory Factor Level in Patients with Coronary Heart Disease Complicated with Helicobacter Polori Infection

作　　者：高建华 何颖辉 GAO Jian-hua;HE Ying-hui(District Hospital of Traditional Chinese and Western Medicine in Hangzhou City,,Hangzhou 310000,China)

机构地区：[1]杭州市西湖区中西医结合医院

出　　处：《现代检验医学杂志》2019年第4期53-57,共5页Journal of Modern Laboratory Medicine

摘　　要：目的探讨冠心病(CHD)并发幽门螺杆菌(HP)感染者血清炎性因子水平和HP抗体谱特征。方法收集2016年12月~2017年12月来院就诊的CHD和健康体检者的血清样本524例,分别设为CHD组(疾病组)296例与非CHD组(对照组)228例,采用血清HP抗体进行检测,筛选CHD并发HP感染患者,另采用化学发光法和免疫印迹法对相应的炎性因子和HP血清抗体谱进行检测,比较两组人群各指标间的差异性。结果在两组人群中疾病组的HP抗体阳性率(52.03%,154/296)高于对照组(35.09%,80/228),差异具有统计学意义(χ^2=14.95,P<0.05),疾病组各炎性因子含量均高于对照组,两组间差异均具有统计学意义(t=-33.34~19.96,均P<0.05);疾病组并发HP感染与对照组的抗体谱分析发现,VacA-95KD,VacA-91KD,UreA-66KD和UreB-30KD抗体在两组间的阳性率差异均无统计学意义(χ^2=0.22~3.49,均P>0.05),而疾病组并发HP感染患者血清CagA-116KD抗体阳性率(76.62%,118/154)高于对照组中HP感染者(62.5%,50/80),且两组间的差异具有统计学意义(χ^2=5.19,P<0.05),疾病组中并发HP感染者血清I型阳性率(76.62%,118/154)高于对照组并发HP感染者(62.5%,50/80),两组间差异具有统计学意义(χ^2=5.19,P<0.05)。疾病组按照HP感染血清型间及与非感染患者血清因子间比较发现,I型血清型血清超敏C反应蛋白(hs-CRP)、同型半胱氨酸(Hcy)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的含量高于II型血清型,且II型血清型hs-CRP,TNF-α,IL-6和Hcy的含量高于非HP感染CHD患者,各组间差异均具有统计学意义(F=524.8~2 829.29,均P<0.05)。结论该文对于CHD患者并发HP感染的血清学特征进行调查,验证了CHD发生于HP感染之间的关联性,积累了HP感染的流行病学资料,为进一步研究CHD的发生、发展以及治疗奠定了科学依据。Objective To investigate the levels of serum inflammatory factors and the characteristics of HP antibody spectrum in patients with coronary heart disease(CHD) complicated with Helicobacter pylori(HP).Methods 524 serum samples were collected from CHD patients and health examinees from December 2016 to December 2017,296 in CHD group and 228 in non-CHD group.Serum HP antibody was used to screen patients with CHD complicated with HP infection.The corresponding inflammatory factors and HP serum antibody spectrum were detected by chemiluminescence and immunoblotting,respectively.The differences of two groups were compared among population indices.Results The positive rate of HP antibody in the two groups(52.03%,154/296) was higher than that in the control group(35.09%,80/228).The difference was statistically significant(χ^2=14.95,P<0.05).The content of inflammatory factors in the disease group was higher than that in the control group, and the difference was statistically significant(t=-33.34^-19.96,all P< 0.05).The antibody analysis of patients of HP infection in the disease group and the control group showed no statistically significant difference in the positive rate of VacA-95 KD,VacA-9 KD,UreA-66 KD and UrteB-30 KD antboy between the two groups(χ^2=0.22~3.49,all P<0.05).The positive rates of serum CagA-116 KD antibodies in patients with HP infection in the disease group(76.62%,118/154) were higher than those in the control group(62.5%,50/80),and the difference between the two groups was statistically significant(χ^2=5.19,P<0.05).The positive rates of serum type I antibodies in patients with HP infection in the disease group(76.62%,118/154) were higher than those in the control group(62.5%,50/80).The difference between the two groups was statistically significant(χ^2=5.19,P<0.05).118/154 were higher than those in the control group(62.5%,50/80).There was a significant difference between the two groups(χ^2=5.19,P<0.05).According to the comparison between serotypes of HP infection and serum factors of non-HP infe

关 键 词：冠心病 幽门螺杆菌 血清幽门螺杆菌抗体 血清抗体谱 

分 类 号：R541.4[医药卫生—心血管疾病] R392.11[医药卫生—内科学]