山东省猪瘟Erns基因系统进化分析与表达模式的研究  

Phylogenetic analysis and expression of the Erns gene of classical swine fever virus from the Shandong Province of eastern China

作　　者：兰邹然[1] 张月[1] 楚遵锋 徐淑华[1] 田夫林[1] 王金宝[2] LAN Zou-ran;ZHANG Yue;CHU Zun-feng;XU Shu-hua;TIAN Fu-lin;WANG Jin-bao

机构地区：[1]山东省动物疫病预防与控制中心,山东济南250022 [2]山东省农业厅,山东济南250002

出　　处：《中国兽医杂志》2019年第4期7-11,共5页Chinese Journal of Veterinary Medicine

基　　金：山东省优秀青年科学家科研奖励基金资助项目(BS2010NY020)

摘　　要：采用RT-PCR方法从山东省采集的怀疑携带猪瘟病毒(CSFV)的病料组织中扩增出全长为696bp囊膜糖蛋白ErnscDNA,其编码232个氨基酸的蛋白质,命名为SDCQ。通过构建E^rns蛋白家族系统进化树分析可知,山东省内流行的CS-FV毒株与传统强毒株和疫苗用毒株在主要抗原编码基因上存在较大差异。将阳性重组表达质粒pPIC9K/E^rns电转化GS115酵母菌,经筛选、鉴定、甲醇诱导表达,SDS-PAGE电泳分析,在培养液上清中检测到了目的蛋白E^rns,大约50kDa。Western Blot显示,E^rns是以同源二聚体的形式存在,且表达产物可以特异性识别CSFV抗体。The envelope glycoprotein region, E rns of the classical swine fever virus (CSFV) was amplified by RT - PCR and se- quenced directly from the sample collected from the Shandong province. Phylogenetic comparisons with 36 reference strains based on the nucleotide sequencing resulted into a phylogenetic tree clustered into two groups of approximately equal sizes. The Shandong strain, designated as SDCQ, did not cluster with other major reference strains from China and outside. SDS - PAGE profiling yielded a single protein band of ~50 kDa. Dimerization of the purified E rns protein was present and was further confirmed by Western blot- ting.

关 键 词：猪瘟 Erns基因 进化树 表达 

分 类 号：S852.651[农业科学—基础兽医学]