福建省谱系3 PRRSV的分离及其ORF5基因序列分析  

Isolation and identification of lineage 3 PRRSV and sequence analysis of ORF5 gene

作　　者：魏春华 夏伟 李娜 马英 吴熠丹 虞慧云 戴爱玲 杨小燕 刘建奎 WEI Chun-hua;XIA Wei;LI Na;MA Ying;WU Yi-dan;YU Hui-yun;DAI Ai-ling;YANG Xiao-yan;LIU Jian-kui(College of Life Sciences of Longyan University , Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology(Long Yan University), Fujian Engineering ResearchCenter for Swine Disease Control and Prevention , Longyan 364000 , China)

机构地区：[1]龙岩学院生命科学学院福建省家畜传染病防治与生物技术重点实验室福建省生猪疫病防控工程技术研究中心

出　　处：《中国兽医杂志》2019年第4期20-24,I0002,共6页Chinese Journal of Veterinary Medicine

基　　金：福建省科技引导项目(2018N0022);福建省教育厅中青年项目(JAT170571);龙岩学院产学研项目(LC2016010)

摘　　要：对福建省新流行谱系3的PRRSV进行分离,并对其分子生物学特性进行初步研究,为新流行毒株的防治提供依据和参考。分离的毒株通过RT-PCR和IFA对PRRSV分离毒株进行鉴定,并对其ORF5基因进行序列测定分析。结果显示,分离株能够在Marc-145细胞上增殖并产生典型的细胞病变,被抗PRRSV的特异性单克隆抗体所识别,具有特异性免疫荧光,其TCID50为105.3,将其命名为FJFQ1805。分离株ORF5基因与北美型PRRSV参考株核苷酸同源性为83.9%~92.2%,与谱系3病毒株QYYZ的同源性最高,为92.2%。系统进化树分析表明,该分离株属于北美型,与QYYZ、GM2和FJFS等谱系3的病毒株属于同一分支。综上,所分离的FJFQ1805毒株为谱系3的病毒株。PRRSV strain of the new epidemic lineage 3 in Fujian Province was isolated using Marc-145 cells and its biological characteristics were preliminarily studied, providing the basis and reference for the prevention and control of new epidemic strains. The virus isolate was determined by RT-PCR and confirmed by immunofluorescence assay (IFA), and the ORF5 gene was sequenced and analyzed. The results showed that PRRSV typical CPE was observed on Marc-145 cells and it was detected by anti-PRRSV spe- cific monoclonal antibodies by IFA,The TCID 50 of cultured virus was 10 5. 3 . The PRRSV strain was named as FJFQ1805. Sequence a- nalysis of ORF5 showed that the isolate shared 83. 9%~92. 2% homology with reference isolates,and a high identity of 99. 2% with QYYZ. The phylogenetic tree analysis showed that the isolate belonged to NA and is closely related to FJFS, QYYZ and GM2. In conclusion, FJFQ1805 belongs to lineage 3 PRRSV.

关 键 词：PRRSV 谱系3 分离鉴定 ORF5基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]