禽流感H5、H7和H9亚型多重PCR方法的建立  

Simultaneous typing of H5, H7 and H9 subtypes of Avian Influenza virus by multiplex PCR

作　　者：胡凌 张鹏 陈有才 张军 戴悦涵 胡卫杰 HU Ling;ZHANG Peng;CHEN You-cai;ZHANG Jun;DAI Yue-han;HU Wei-jie(Animal Epidemic Prevention and Control Center of Zigong city , Zigong 643000 , China)

机构地区：[1]四川省自贡市动物疫病预防控制中心,四川自贡643000

出　　处：《中国兽医杂志》2019年第4期40-43,共4页Chinese Journal of Veterinary Medicine

摘　　要：为了建立能够同时检测禽流感H5、H7和H9亚型的多重PCR检测方法,本文分别针对各病毒亚型的HA基因高度保守序列设计了3对特异性引物。通过对反应条件的优化,成功建立了在一个反应中同时检测禽流感H5、H7和H9亚型的多重PCR诊断方法。根据其敏感性的结果可知,该方法对这3种亚型病毒的最低核酸检测量均为1×10^4拷贝/μL,且该方法特异性良好,具有灵敏、快速、成本低等优点,对禽流感病毒的流行病学调查具有指导意义。To establish a multiplex PCR for simultaneously typing H5, H7 and H9 subtypes of Avian Influenza,three specific primers were designed for the highly conservative sequence of the HA gene. After optimizing reaction conditions, a multiplex PCR for simultaneously typing of H5,H7 and H9 subtypes of Avian Influenza was successfully established. The sensitivity of the multiplex PCR was 1 ×10^4 copies /μL for H5,H7,and H9. This method is a rapid,sensitive,specific and cost-effective diagnostic tool for single and mixed infections. And it has guiding significance for diagnosis and epidemiological investigation of reproductive pathogens in avian influenza virus.

关 键 词：多重PCR 禽流感 H5 H7 H9 

分 类 号：Q939.47[生物学—微生物学]