半胱亚磺酸脱羧酶在妊娠小鼠子宫和胚胎中的表达定位研究  

Study on expression and localization of CSD in the uterus and embryo of mouse

作　　者：李炜 吕钊旭 冯永珍 张淑芝[4] 左语馨 范晶晶[4,5] LI Wei;LYU Zhaoxu;FENG Yongzhen;ZHANG Shuzhi;ZUO Yuxin;FAN Jingjing(Department of Internal Medicine,Weifang Nursing Vocational College,Weifang, Shandong 261041,China;Department of Neonatology,Weifang Maternity and Child Care Hospital,Weifang,Shandong 261000,China;Department of Nursing,School Hospital of Weifang University,Weifang,Shandong 261061,China;College of Biological and Agricultural Engineering, Weifang University,Weifang,Shandong 261061,China;Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong/Weifang University,Weifang,Shandong 261061,China)

机构地区：[1]潍坊护理职业学院内科教研室,山东潍坊261041 [2]山东省潍坊市妇幼保健院新生儿科,261000 [3]潍坊学院校医院护理部,山东潍坊261061 [4]潍坊学院生物与农业工程学院,山东潍坊261061 [5]山东省高校生物化学与分子生物学重点实验室/潍坊学院,山东潍坊261061

出　　处：《重庆医学》2019年第14期2349-2352,共4页Chongqing medicine

基　　金：国家星火计划项目(2015GA740060);山东省自然科学基金项目(ZR2016CB28,BS2013SW036);山东省莱州湾海洋动物健康养殖工程技术协同创新中心项目(CXGX15005PT);山东省潍坊市科学技术发展计划项目(2016GX022,2017GX005)

摘　　要：目的研究半胱亚磺酸脱羧酶(CSD)mRNA和蛋白在妊娠小鼠子宫和胚胎中的表达定位。方法构建CSD原位杂交探针,收集正常妊娠小鼠第1~9天子宫组织和第11天胚胎,采用免疫组织化学染色、原位杂交检测CSD的表达情况。结果小鼠妊娠第1~2天CSD主要定位在子宫腔上皮细胞和腺上皮细胞,基质细胞从第3天开始表达,第6天开始胚胎周围的子宫蜕膜细胞中检测不到CSD蛋白,第8天子宫CSD的表达明显减弱,第9天表达更弱。从囊胚到第9天时胚胎都有CSD表达。CSD在第7天定位于着床点子宫和胚胎中,胚胎周围的子宫蜕膜细胞无表达,在第11天定位于胚胎的脑、心脏、脊髓。结论子宫和胚胎在小鼠妊娠过程中可表达CSD。Objective To explore the expression and localization of cysteine sulfinate decarboxylase(CSD)in mouse uterus and embryo during pregnancy.Methods CSD in situ hybridization probes were constructed,then collected the uterus of pregnant mice from the first day to the ninth day and the embryos on the eleventh day,the expression of CSD was detected by immunohistochemistry and in situ hybridization.Results The epithelial and gland cells of uterus were positive for CSD from the first day to the second day,the stromal cells became intensely positive for CSD from the third day.CSD protein was not detected in decidual cells around embryo from the sixth day.The CSD response in the uterus was significantly weakened on the eighth day,and the expression was even weaker on the ninth day.The embryo expressed CSD from the blastocyst to the embryo on the ninth day.Uterus and embryo were positive for CSD whereas the decidual cells in the implantation site were unstained on the seventh day,CSD was positive at brain,heart and spinal cord in the embryo on the eleventh day.Conclusion CSD expresses in the uterus and embryo of the pregnant mice.

关 键 词：半胱亚磺酸脱羧酶 子宫 胚胎 哺乳动物 定位 

分 类 号：Q813.7[生物学—生物工程]