刺山柑总生物碱对系统性硬皮病HGF/C-Met通路的调控作用  

Effects of capparis spinosa total alkaloid on hepatocyte growth factor/C-Met signalling pathways in systemic sclerosis

作　　者：康小龙[1] 何承辉 卢军[1] KANG Xiaolong;HE Chenghui;LU Jun(Department of Medicine Research,Hospital of Chinese Medicine Affiliated to Xinjiang Medical University,Urumqi,Xinjiang 830000,China;Department of Pharmaceutics,Xinjiang Medicine Research Institute,Urumqi,Xinjiang 830000,China)

机构地区：[1]新疆医科大学附属中医医院药研室,乌鲁木齐830000 [2]新疆维吾尔自治区药物研究所药剂室,乌鲁木齐830004

出　　处：《重庆医学》2019年第14期2353-2355,2359,共4页Chongqing medicine

基　　金：国家自然科学基金项目(81760560)

摘　　要：目的研究刺山柑总生物碱对系统性硬皮病(SSc)肝细胞生长因子(HGF)/C-Met通路的调控作用。方法90只雌性BALB/c小鼠分为对照组,SSc模型组,低(225mg/kg)、中(450mg/kg)、高(900mg/kg)剂量刺山柑总生物碱组及青霉胺组。小鼠皮下注射博莱霉素4周建立SSc模型后,刺山柑总生物碱组背部外敷刺山柑总生物碱乳膏,青霉胺组给予青霉胺灌胃,正常组、SSc模型组背部外敷不含药乳膏基质,每天1次,连续8周。Western blot检测皮肤组织HGF表达,ELISA实验检测小鼠皮肤组织HGF和C-Met水平。结果中、高剂量刺山柑总生物碱组小鼠HGF和C-Met表达较SSc模型组明显降低(P<0.05)。结论刺山柑总生物碱可能通过上调HGF/C-Met通路改善SSc组织纤维化。Objective To explore the effects of capparis spinosa total alkaloid on hepatocyte growth factor(HGF)/C-Met signalling pathways in systemic sclerosis(SSc).Methods A total of 90 female BALB/c mice were divided into the control group,the SSc model group,the low-(225 mg/kg),medium-(450 mg/kg)and high-dose(900 mg/kg)capparis spinosa total alkaloid groups and the penicillamine group.Subcutaneous injection of bleomycin was injected in mice for 4 weeks to establish SSc model.The capparis spinosa total alkaloid groups received external application with capparis spinosa total alkaloid cream,the penicillamine group were given penicillamine for gavage,the control group and the model group received external application without substance with once a day for 8 weeks.The expression of HGF in skin tissue was detected by Western blot and the levels of HGF and C-Met in skin tissue were measured by ELISA.Results Compared with the SSc model group,the expressions of HGF and C-Met significantly decreased more in the medium-and high-does capparis spinosa total alkaloid groups(P<0.05).Conclusion Capparis spinosa total alkaloid is effective in treating fibrosis of SSc by up-regulating HGF/C-Met signalling pathways.

关 键 词：刺山柑总生物碱 硬皮病 系统性 纤维化 肝细胞生长因子 C-MET 

分 类 号：R965.1[医药卫生—药理学]