牛蒡苷元及牛蒡子提取物的急性毒性试验研究  

Study on Acute Toxicity of Arctiin and Burdock Seed Extract

作　　者：何斌 冉志平 周琦 张海静 杨文海 邵志勇 吴利军 陈夏冰 陈洁 操继跃[2] HE Bin;RAN Zhi-ping;ZHOU Qi;ZHANG Hai-Jing;YANG Wen-Hai;SHAO Zhi-Yong;WU Li-Jun;CHEN Xia-Bing;CHEN Jie;CAO Ji-Yue(Institute of Animal Husbandry and Veterinary,Wuhan Academy of Agricultural Sciences,Wuhan 430208,China;College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;Tianjin baodi district animal husbandry and aquaculture development service center,Tianjin 301800,China)

机构地区：[1]武汉市农业科学院畜牧兽医研究所,武汉430208 [2]华中农业大学,武汉430070 [3]天津市宝坻区畜牧水产业发展服务中心,天津301800

出　　处：《中国兽药杂志》2019年第7期44-48,共5页Chinese Journal of Veterinary Drug

摘　　要：为评价牛蒡苷元及牛蒡子提取物在兽医临床使用中的安全性,根据兽药急性毒性试验(LD50测定)指导原则的要求,开展了牛蒡苷元及牛蒡子提取物在SPF级昆明小鼠经口给药的急性毒性试验研究。试验结果表明:小鼠灌胃给予牛蒡苷元的半数致死量为219.8mg/kg,死亡均发生在3h以内,存活动物均正常生长;鼠灌胃给予牛蒡子提取物后采用最大耐受量法测定发现,给药组与对照组雌雄小鼠生长良好,在14d观察期内均未出现明显中毒症状和死亡情况,药物急性经口MTD≥36g/kg.bw;根据兽药指导原则经口给药急性毒性试验剂量分级标准,牛蒡苷元属中等毒级,牛蒡子提取物为实际无毒级。In order to evaluate the safety of arctiin and fructus lappa lappa extract in veterinary clinical use,the acute toxicity test of arctiin and fructus lappa extract in SPF grade kunming mice was conducted according to the requirements of the veterinary acute toxicity test( LD50 test) guidelines. The results showed that the lethal dose of arctium lappa was 219.8 mg/kg,all the deaths occurred within 3 h,and all the surviving animals grew normally.After the mice were given burdock extract by gavage,the method of maximum tolerance was adopted. It was found that both male and female mice in the administration group and the control group grew well,and no obvious poisoning symptoms or death occurred during the 14 d observation period. According to the veterinary drug guidelines for oral administration of acute toxicity test dose grading standards,burdock glycoside is of medium toxic grade,and burdock seed extract is actually non-toxic grade.

关 键 词：牛蒡苷元 牛蒡子提取物 昆明小鼠 急性毒性 

分 类 号：S853.74[农业科学—临床兽医学]