卡泊芬净对粪肠球菌生物膜形成能力影响的初步研究  

Preliminary study on effect of caspofungin on biofilm forming ability of Enterococcus faecalis

作　　者：白冰[1] 林志伟[1] 徐广健 孙翔 陈俊文 涂浩鹏 李多云[1] 余治健[1] 邓启文[1] 郑金鑫[1] BAI Bing;LIN Zhi-wei;XU Guang-jian;SUN Xiang;CHEN Jun-wen;TU Hao-peng;LI Duo-yun;YU Zhi-jian;DENG Qi-wen;ZHENG Jin-xin(Department of Infectious Diseases and Shenzhen Key Laboratory for Endogenous Infection,Shenzhen Nanshan Hospital of Shenzhen University,Shenzhen 518052,China)

机构地区：[1]深圳大学附属深圳市南山区人民医院深圳市内源性感染诊治研究重点实验室

出　　处：《中国感染控制杂志》2019年第7期607-611,共5页Chinese Journal of Infection Control

基　　金：深圳市科技创新委基础研究基金项目(JCYJ20170307153714512);深圳市南山区科技计划项目(2018025)

摘　　要：目的分析卡泊芬净抑制粪肠球菌生物膜形成的情况。方法选取临床来源生物膜强阳性粪肠球菌1株及不同生物膜形成能力的粪肠球菌菌株10株,同时设卡泊芬净干预组和对照组,干预组加卡泊芬净,浓度为20μmol/L,对照组加生理盐水。采用结晶紫染色法,通过酶标仪检测两组粪肠球菌浮游菌生长及生物膜形成情况。结果卡泊芬净对粪肠球菌临床株的浮游菌生长无抑制作用,可显著抑制菌株生物膜形成。结论卡泊芬净对粪肠球菌生物膜形成能力有显著抑制作用。Objective To analyze the effect of caspofungin on inhibiting the formation of Enterococcus faecalis(E.faecalis) biofilm. Methods One strain of clinically isolated E.faecalis with strong positive biofilm and 10 strains of E.faecalis with different biofilm forming ability were selected,caspofungin intervention group and control group were set up at the same time,intervention group used caspofungin with the concentration of 20 μmol/L,control group used normal saline.Growth of planktonic strains and biofilm formation of E.faecalis in two groups were detected by crystal violet staining and microplate reader. Results Caspofungin had no inhibitory effect on the growth of planktonic clinical E.faecalis strains,but can significantly inhibit the formation of biofilm. Conclusion Caspofungin has a significant inhibitory effect on biofilm formation of E.faecalis.

关 键 词：卡泊芬净 生物膜 粪肠球菌 

分 类 号：R446.5[医药卫生—诊断学]