肉桂醛促进子宫内膜癌RL95-2细胞凋亡的机制  

Mechanism of Cinnamaldehyde Promoting the Apoptosis of RL95-2 Cells in Endometrial Carcinoma

作　　者：陈立平[1] 王柏欣[2] 王景涛[2] 符婕[1] 魏宏[1] 张东东[2] 刘蕾[2] CHEN Li-ping;WANG Bai-xin;WANG Jing-tao;Fu Jie;WEI Hong;ZHANG Dong-dong;LIU Lei(Jiamusi Center Hospital;School of Basic Medicine of Jiamusi University,Jiamusi 154007,China)

机构地区：[1]佳木斯市中心医院妇产科 [2]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《中山大学学报（医学版）》2019年第4期540-545,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：黑龙江省卫生厅资助项目(2007-140)

摘　　要：【目的】探讨肉桂醛对子宫内膜癌RL95-2细胞凋亡的影响及其作用机制。【方法】选取肉桂醛作用子宫内膜癌RL95-2细胞,噻唑蓝(MTT)比色法检测子宫内膜癌RL95-2细胞的增殖活性和肉桂醛的IC50,Hoechst33258染色荧光显微镜观察细胞凋亡形态学改变,流式细胞术检测RL95-2细胞的凋亡百分率,Western blot检测肉桂醛对RL95-2细胞Cleaved caspase-3、Caspase-3、NF-κB、IL-6和IGF-R蛋白表达的影响。【结果】肉桂醛可降低子宫内膜癌RL95-2细胞的增殖率,与药物浓度和作用时间有关;与溶剂对照组比较,肉桂醛组(0.29、0.59和1.20mg/mL)作用48h后RL95-2细胞的凋亡百分率明显增高(P<0.01),出现典型的凋亡小体,Cleaved caspase-3蛋白的表达明显增加(P<0.01),Caspase-3蛋白的表达无明显变化(P>0.05),而NF-κBp65、IL-6和IGF-R蛋白的表达均明显降低(P<0.05)。【结论】肉桂醛可降低RL95-2细胞NF-κB·p65、IL-6和IGF-R蛋白的表达,促进RL95-2细胞的凋亡,从而起到抗子宫内膜癌作用。【Objective】To investigate the effect of cinnamaldehyde on the apoptosis of RL95-2 cell in endometrial carcinoma.【Methods】Endometrial carcinoma RL95-2 cells were treated with cinnamaldehyde,and the proliferation activity and IC50 of endometrial carcinoma RL95-2 cells were detected by MTT colorimetry assay.Apoptotic morphology was observed after Hoechst 33258 staining.The percentage of apoptosis in RL95-2 cells was measured by flow cytometry. Western blot analysis was used to test the effect of cinnamaldehyde on the expression of Cleaved caspase-3,caspase-3, NF-κB·p65,IL-6 and IGF-R in RL95-2 cells.【Results】Cinnamaldehyde can reduce the viability rate of endometrial cancer RL95-2 cells,which is related to the treatment duration and concentration.Compared with the solvent control group,the apoptosis percentage of RL95- 2 cells in the cinnamaldehyde group (0.29,0.59,1.20 mg/mL) was significantly increased after 48 hours(P < 0.01),typical apoptotic bodies were found,and the expression of Cleaved caspase-3 protein was significantly increased(P < 0.01),there was no significant change in the expression of Caspase 3 protein(P > 0.05),while the expression of NF-κB·p65,IL-6 and IGF-R protein were significantly increased(P < 0.05).【Conclusion】Cinnamaldehyde can reduce the expression of NF-κB·p65,IL-6 and IGF-R proteins in RL95-2 cells and promote the apoptosis of RL95-2 cells,thus playing an anti-endometrial cancer role.

关 键 词：肉桂醛 RL95-2 NF-ΚB IL-6 IGF-R 

分 类 号：R711[医药卫生—妇产科学]