小麦DUS测试已知品种DNA指纹数据库构建及其应用  

Construction of DNA Profile Database of Wheat Reference Varieties and Its Application in Wheat DUS Test

作　　者：郑永胜[1] 张晗[1] 王雪梅[1] 王东建[1] 孙加梅[1] 王穆穆 王晖 王玮[1] 李华[1] 段丽丽[1] 李汝玉[1] ZHENG Yong-sheng;ZHANG Han;WANG Xue-mei;WANG Dong-jian;SUN Jia-mei;WANG Mu-mu;WANG Hui;WANG Wei;LI Hua;DUAN Li-li;LI Ru-yu(Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100)

机构地区：[1]山东省农业科学院作物研究所

出　　处：《植物遗传资源学报》2019年第4期845-853,共9页Journal of Plant Genetic Resources

基　　金：山东省农业科学院农业科技创新工程(CXGC2017A02);山东省农业科学院青年基金(2015YQN14)~~

摘　　要：为解决DUS测试中特异性测试近似品种筛选难的问题,本研究基于2256份DUS测试小麦已知品种构建DNA指纹数据库。首先利用81对SSR引物对96份小麦品种进行遗传相似度分析。结果表明,81对SSR引物扩增出84个位点,共检测到731个等位变异,每个位点的等位变异数在3~18之间,多态性信息指数(PIC)变化范围为0.29~0.89。选取42个SSR标记构建了2256份小麦品种DNA指纹数据库,共获得148493个数据,除引物barc14、xgwm341之外,其他引物数据缺失率在5%以内。对176份小麦品种进行DNA指纹数据采集和特异性测试,结果表明不具有特异性的申请品种与其最近似品种的遗传相似度都在90%以上;本研究基于42对SSR荧光标记引物构建了我国小麦DUS测试已知品种的DNA指纹数据库,确定近似品种筛选的遗传相似度阈值为80%,建议将与申请品种遗传相似度高于80%的已知品种作为近似品种进行田间种植和特异性评价。Based on 2256 common wheat reference varieties for DUS test in China,a DNA profile database was established to assist in distinctness test.We first analyzed the DNA profiles of 96 reference varieties using 84fluorescently labeled SSR markers.A total of 731 alleles were detected with the number of alleles varying between3 and 18 and the PIC values from 0.29 to 0.89.A DNA profile database consiting of 2256 wheat reference varieties was constructed using 42 fluorescently labeled SSR markers.A total of 148493 datapoint were included with a missing rate of less than 5% for each maker except makers barc14 and xgwm341.The genetic and genotypic datasets of 176 wheat varieties were collected and analyzed.The genetic similarities between the non-distinct candidate varieties and their most similar vairieties were above 90%.The candidate varieties with a genetic similarity of over 80% are assumed to be similar varieties,which are suggested to be further investigated for phenotypic variations in the field.

关 键 词：DUS测试 已知品种 特异性测试 SSR标记 DNA指纹库 

分 类 号：S512.1[农业科学—作物学]