河北省12个小麦主栽品种(系)抗叶锈性鉴定及基因分析  

Identification and Analysis of Leaf Rust Resistance Genes in 12 Main Wheat Cultivars(Lines)from Hebei Province

作　　者：张晓玲 张换换[1] 徐新玉 杨华丽[1] 段振盈 姚占军[1] ZHANG Xiao-ling;ZHANG Huan-huan;XU Xin-yu;YANG Hua-li;DUAN Zhen-ying;YAO Zhan-jun(College of Agronomy,Agricultural University of Hebei/North China Key Laboratory for Germplasm Resources of China Education Ministry,Baoding 071001)

机构地区：[1]河北农业大学农学院/华北作物种质资源研究与利用教育部重点实验室

出　　处：《植物遗传资源学报》2019年第4期982-990,共9页Journal of Plant Genetic Resources

基　　金：国家重点研发计划(2017YFD300901)~~

摘　　要：为明确河北省12个小麦主栽品种(系)的抗叶锈性及抗叶锈基因,在苗期选用20个不同毒性谱的小麦叶锈菌菌系接种12个小麦品种(系)以及37个含有已知抗叶锈病基因的载体品种以进行基因推导,同时利用11个与已知抗叶锈病基因紧密连锁的分子标记对12个品种(系)进行标记检测。为进一步鉴定成株期抗性,2014-2015年和2015-2016年连续2年分别在河北保定和河南周口对供试材料进行田间严重度调查。结果表明,在12个品种(系)中检测到Lr1、Lr26、LrB和Lr46共4个抗叶锈病基因,其中8个品种(系)推测含有Lr26,石新618可能含有Lr1,藁优2018可能含有LrB,冀5265可能含有Lr46。2年2点的田间鉴定结果表明,石新733、藁优2018和石优17为慢锈品种(系),可作为抗源材料加以利用。In order to identify the resistance and leaf rust resistance genes of 12 wheat cultivars from Hebei province,we conducted tests for leaf rust resistance using 20 Puccinia triticina Erikss.pathotypes at the seedling stage of 12 wheat cultivars(lines) as well as 37 cultivars(lines),which are the resistant donors with known leaf rust resistance genes.Meanwhile,marker-assisted identification using 11 molecular markers closely linked were performed.In order to identify the adult plant leaf rust resistance,the diseases severity of the tested materials was investigated by field trails in 2014-2015 and 2015-2016 cropping seasons at Zhoukou,Henan Province and Baoding,Hebei Province.Four leaf rust resistance genes including Lr1,Lr26,LrB and Lr46,were detected in 12 wheat cultivars(lines),and eight cultivars(lines) may carry Lr26.These cultivars Shixin 618,Gaoyou 2018 and Ji 5265 may carry Lr1,LrB,Lr46 respectively.As indicated by field identification in two field growing seasons,Shixin 733,Gaoyou 2018 and Shiyou 17 showed slow delayed establishment of leaf rust disease symptom rusting,and they can be used as the resistance donors for breeding.

关 键 词：小麦叶锈病 基因推导 分子检测 成株抗性 

分 类 号：S435.121.43[农业科学—农业昆虫与害虫防治]