大豆E3泛素连接酶基因GmAIRP1的同源克隆及在烟草中的功能鉴定  

Homologous Cloning of Soybean E3 Ubiquitin Ligase Gene GmAIRP1 and Its Functional Identification in Tobacco

作　　者：张雅文 沈祥娟 张静 朱美娇[1] 张海玲[2] 王全伟[1] ZHANG Ya-wen;SHEN Xiang-juan;ZHANG Jing;ZHU Mei-jiao;ZHANG Hai-ling;WANG Quan-wei(College of Life Science and Technology,Harbin Normal University/Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province,Harbin 150025;Institute of Grass Research,Heilongjiang Academy of Agriculture Sciences,Harbin 150086)

机构地区：[1]哈尔滨师范大学生命科学与技术学院/黑龙江省分子细胞遗传与遗传育种重点实验室,哈尔滨150025 [2]黑龙江省农业科学院草业研究所,哈尔滨150086

出　　处：《植物遗传资源学报》2019年第4期1011-1019,共9页Journal of Plant Genetic Resources

基　　金：哈尔滨市科技创新人才研究专项(RC2013 QN002103);黑龙江省博士后资助项目(LBH-Z13185);黑龙江省教育厅科学技术研究项目(12511154);哈尔滨师范大学博士科研启动基金(KGB200903)~~

摘　　要：E3泛素连接酶在植物抵御高盐及干旱等非生物胁迫过程中发挥重要作用。本研究克隆获得大豆E3泛素连接酶基因GmAIRP1,该基因cDNA全长为642 bp,编码213个氨基酸。蛋白结构域分析表明,GmAIRP1具有典型的RING-finger结构域。系统进化树分析表明,GmAIRP1与蒺藜苜蓿MtAIRP1同源性最高,亲缘关系最近。表达分析显示,GmAIRP1可被高盐、干旱和ABA诱导表达,并在胁迫1 h或3 h时表达量达到最大。抗逆表型分析表明,GmAIRP1转基因烟草在高盐和干旱胁迫21 d后,生长状态优于野生型,提高了植株对高盐和干旱胁迫的耐受性。生理指标测定结果显示,在高盐和干旱胁迫下,GmAIRP1转基因烟草的POD和CAT活性提高,整体高于对照,MDA含量始终低于对照。以上研究结果表明,Gm AIRP1能够通过激活抗氧化酶活性、提高渗透调节物质的积累来增强植物抵御高盐和干旱胁迫的能力,在植物响应高盐和干旱胁迫中发挥正调控作用。Plant E3 ubiquitin ligase plays an important role against abiotic stresses such as high salt and drought.In this study,we isolated soybean E3 ubiquitin ligase gene GmAIRP1,which contained a full length coding sequence of 642 bp,encoding for 213 amino acids.Protein domain analysis indicated that GmAIRP1 carried a typical RING-finger domain.By phylogenetic tree analysis,GmAIRP1 was found to be closely related with MtAIRP1.The expression of GmAIRP1 turned to be elevated under salt,drought or ABA stresses,particularly at 1 h or 3 h post treatments.By transforming GmAIRP1 into tobacco,the transgenic tobacco grew better than the wild type after 21 days of high salt and drought stress,implying an elevated stress tolerance.Furthermore,we analyzed the physiological indicators,including POD and CAT activities that were elevated in transgenic plants than that of the control,and the MDA content that was constantly lower in transgenic plants.Taken together,these results indicated that GmAIRP1 acts as a positive regulator in plant responding to high salt and drought stress,possibly by activating antioxidant enzyme activity and increasing accumulation of osmotic adjustment substances.

关 键 词：大豆 E3泛素连接酶 GmAIRP1 表达分析 功能鉴定 

分 类 号：S565.1[农业科学—作物学] Q943.2[生物学—植物学]