商洛黄芩汉黄芩素提取及复合保鲜液应用研究  

Wogonin Extraction from Shangluo Scutellaria baicalensis and Application of Its Compound Preservative Solution

作　　者：张晓虎 李智文 殷佳琦 高佳 Zhang Xiaohu;Li Zhiwen;Yin Jiaqi;Gao Jia(College of Biology Pharmacy and Food Engineering,Shangluo University,Shangluo Shaanxi 726000)

机构地区：[1]商洛学院生物医药与食品工程学院

出　　处：《中国农学通报》2019年第22期147-155,共9页Chinese Agricultural Science Bulletin

基　　金：2016年国家级大学生创新训练项目“黄芩有效成分的提取及抑菌效果的初步探究”(201611396012);2018年国家级大学生创业训练项目“芩芬复方天然食品保鲜剂研发有限责任公司”(201811396002X);商洛市2016年科学技术研究发展计划项目“中国秦岭‘五大商药’系列专用肥配方研制”(SK2016-49)

摘　　要：以商洛黄芩为实验材料,采用超声辅助乙醇提取黄芩中汉黄芩素,研究超声时间、超声提取温度、超声功率、料液比和乙醇体积分数等因素对汉黄芩素提取率的影响。在单因素实验的基础上,设计3因素3水平响应面实验,分析各因素的显著性和交互作用,在优化工艺超声功率140 W、料液比1:20、乙醇体积分数60%前提下,黄芩汉黄芩素提取率为0.526%。将汉黄芩素提取液与1.5%壳聚糖、1%柠檬酸进行复配,制备复合保鲜液,以感官指标、pH值、菌落总数为检测指标,模拟易导致新鲜草莓变质的温度和湿度进行保藏实验,通过对草莓品质的评价说明不同浓度黄芩汉黄芩素复合保鲜液的保鲜效果,结果表明0.20%复合保鲜液即可以保藏新鲜草莓3天,0.40%复合保鲜液能够有效地保藏新鲜草莓5天。由于实验模拟草莓极易腐败变质的温度,可以预测室温下草莓贮藏期更长。本研究结果可作为商洛地区黄芩中汉黄芩素应用的技术参考。Shangluo Scutellaria baicalensis was used as the experimental material to extract wogonin by ultrasonic assisted ethanol.The effects of ultrasonic time,ultrasonic temperature,ultrasonic power,material to liquid ratio and ethanol concentration on the wogonin yield were studied.On the basis of single factor experiments,3 factors and 3 levels response surface tests were designed to analyze the significance and correlations of the factors.The optimized process was as follows:the extraction rate of wogonin was 0.526%under the condition of ultrasonic power 140 W,material to liquid ratio was 1:20,and ethanol concentration was60%.The extract of wogonin was mixed with 1.5%chitosan and 1%citric acid to prepare compound preservative liquid.The temperature and humidity of fresh strawberry were simulated with sensory index,pH value and the total number of colonies as the test indexes.Through the evaluation of strawberry quality,the effects of different concentrations of S.baicalensis wogonin compound preservative solution were studied.The results showed that:the preservation time of 0.20%S.baicalensis wogonin compound preservative solution could preserve fresh strawberry for 3 d,and the 0.40%S.baicalensis wogonin compound preservative solution could effectively preserve fresh strawberry for 5 d.Because the simulated temperature is the easy decay one for strawberry,the strawberry can be predicted to have longer storage time at room temperature.The study can be used as a reference for the application of S.baicalensis wogonin in Shangluo.

关 键 词：黄芩 汉黄芩素 超声辅助 乙醇 响应面法 

分 类 号：S188[农业科学—农业基础科学]