基于转录组测序技术筛选花■卵巢发育相关基因  

Transcriptome analysis of spotted steed(Hemibarbus maculatus Bleeker) to identify genes related to ovary development

作　　者：刘慧芬 卢良盛 王静 周传江 顾钱洪 马晓 冯军厂 聂国兴 李学军 LIU Huifen;LU Liangsheng;WANG Jing;ZHOU Chuanjiang;GU Qianhong;MA Xiao;FENG Junchang;NIE Guoxing;LI Xuejun(Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation,College of Fisheries,Henan Normal University,Xinxiang 453007,China)

机构地区：[1]河南师范大学水产学院河南省水产动物养殖工程技术研究中心

出　　处：《水产学报》2019年第8期1714-1722,共9页Journal of Fisheries of China

基　　金：河南省创新型科技团队支持计划(CXTD2016043);河南省科技攻关重点项目(162102110147;182102110383);农业农村部热带亚热带水产资源利用与养殖重点实验室开放课题;河南师范大学博士科研启动课题(qd15186),河南师范大学校青年基金~~

摘　　要：为筛选影响花[鱼骨]卵巢发育相关的基因,采用Illumina Hiseq技术对花[鱼骨]脑、卵巢和肝脏组织进行高通量转录组测序。结果显示,3个组织分别产生了49 484 132、47 540 538和50 622 304个clean reads,组装后共获得了99 878个unigenes,平均长度为1 430 bp。DE seq分析发现,在脑vs.卵巢组中特异性表达的基因数为2 305个,脑vs.肝脏组中特异性表达的基因数为839个,卵巢vs.肝脏组中特异性表达的基因数为1 474个,3个比较组共有的差异表达基因数为860个。GO分析发现,上述差异表达基因主要集中在初级代谢过程(primary metabolic process)、单细胞过程(single-organism process)、有机物代谢过程(organic substance metabolic process)等生物学过程中。KEGG pathway分析显示,一些与卵巢发育和减数分裂相关的信号通路得到了显著富集,如GnRH信号通路、类固醇激素合成、TGF-β信号通路、卵母细胞减数分裂等代谢通路。本研究结果丰富了花[鱼骨]的基因资源,可为花[鱼骨]的繁殖生物学研究提供基础数据。In order to identify candidate genes involved in ovary development,Illumina Hiseq technology was used to define the transcriptome of spotted steed brain,ovary and liver.A total of 49 484 132,47 540 538 and 50 622 304 clean reads were generated from brain,ovary and liver,and then assembled into 99 878 Unigenes with an average length of 1 430 bp.DEseq analysis revealed that 2 305 were found only in the expression of brain vs.ovary group,839 were found only in the expression of brain vs.liver group,1 474 were found only in the expression of ovary vs.liver group,and 860 genes were found to be co-expressed in the three comparison groups.Gene ontology analysis indicated that many of these genes encoded proteins involved in primary metabolic process,singleorganism process,organic substance metabolic process.Based on KEGG pathway analysis,putative genes are novel candidate regulators of oocyte development and meiosis,such as GnRH signaling pathway,Steroid hormone biosynthesis,TGF-beta signaling pathway and Oocyte meiosis.The spotted steed transcriptome data reported here will provide valuable new data to increase genomic resources of this species.The data will also provide a valuable basis for further reproductive molecular biology studies of this commercial species.

关 键 词：花[鱼骨] 卵巢发育 转录组 

分 类 号：Q785S917.4[生物学—分子生物学]