利用微卫星标记分析兴义鸭的遗传多样性  

Genetic diversity of Xingyi duck by using micro-satellite marker

作　　者：李志惠 李杰章 张依裕[2] LI Zhihui;LI Jiezhang;ZHANG Yiyu(Department of Animal Science and Fishery,Guizhou Vocational College of Agriculture,Qingzhen,Guizhou Province 551400,China;College of Animal Science,Guizhou University,Guiyang,Guizhou Province 550025,China)

机构地区：[1]贵州农业职业学院畜牧水产系,贵州清镇551400 [2]贵州大学动物科学学院,贵州贵阳550025

出　　处：《中国饲料》2019年第14期29-32,共4页China Feed

基　　金：国家自然科学基金(31160453);贵州省自然科学基金(黔科合J字[2011]2220号);贵州大学博士基金(贵大人基合字(2010)037号)

摘　　要：文章旨在阐明兴义鸭的遗传多样性,为其保种与开发利0用提供参考依据。采用20对引物扩增兴义鸭的微卫星片段和判型,计算每个微卫星座位的有效等位基因数(Ne)、等位基因频率(P)、基因杂合度(H)及多态信息含量(PIC)。结果显示:20对微卫星引物在兴义鸭群体中均检测到多态,共检测到85个等位基因,平均每个微卫星基因座等位基因数4.2500个,群体平均杂合度H=0.6850,平均多态含量PIC=0.6284,平均有效等位基因数Ne=3.4449个,其中AJ272578、AJ515883、AJ515889、AJ515895、AJ515897、AJ515900表现为中度多态,剩下的14个位点均表现出高度多态性,试验结果表明,在兴义鸭具有丰富的遗传多样性,具有重要的保种价值和选育开发潜能。This study aimed to evaluate the genetic diversity of Xingyi duck,which provides reference for conservation and exploitation.Using 20 pairs of primers Xingyi duck microsatellite fragments and sub-type,was calculated for each microsatellite loci effective number of alleles(Ne),allele frequency(P),genetic heterozygosity(H)and polymorphic information content(PIC).The results showed that:20 pairs of microsatellite primers in Xingyi duck populations were detected polymorphisms which were detected 85 alleles,the average microsatellite locus alleles P=4.2500,group average heterozygosity H=0.6850 the average polymorphic content PIC=0.6284,the average effective number of alleles Ne=3.4449,including AJ272578,AJ515883,AJ515889,AJ515895,AJ515897,AJ515900 showed moderate polymorphism or low polymorphism,and the remaining 14 bits point showed a high degree of polymorphism,experimental results show that in Xingyi duck has a r ich genetic diversity has important conservation value and development potential breeding.

关 键 词：兴义鸭 微卫星 遗传多样性 

分 类 号：S834[农业科学—畜牧学]