依托泊苷脂质体的抗肿瘤活性研究  

Studies on the Anti-tumor Activity of Liposomal Formulations of Etoposide

作　　者：杨芸[1] 黄颖 梁梦艺 李明娟[1] 计燕萍[1] 黄嬛[1] Yang Yun;Huang Ying;Liang Mengyi;Li Mingjuan;Ji Yanping;Huang Xuan(College of Medicine,Jiaxing University,Jiaxing 314001,China)

机构地区：[1]嘉兴学院医学院

出　　处：《广东化工》2019年第14期19-20,共2页Guangdong Chemical Industry

基　　金：浙江省实验动物科技计划项目(2016C37109)

摘　　要：目的:制备依托泊苷载药脂质体,并对其进行细胞药效学评价。方法:依据最佳工艺条件制备依托泊苷脂质体,并通过HPLC方法进行包封率和载药量的评价。将制备好的载药脂质体作用于小细胞肺癌H446细胞和人乳腺癌MCF-7细胞并计算IC50,比较该依托泊苷纳米脂质体对这两种肿瘤细胞的抑制作用。结果:制备得到的载药脂质体的包封率为79.77%,载药量为21.65%。细胞药效学评价实验表明,对H446细胞,游离药物和依托泊苷载药脂质体的IC50分别为2.40μg/mL和1.56μg/mL;对MCF-7细胞的IC50分别为1.28μg/mL和0.35μg/mL。结论:通过应用脂质载体对模型药物依托泊苷进行包裹,对H446和MCF-7两种肿瘤细胞的抑制作用显著增强。Objective:To prepare Etoposide-loaded liposomes based on the optimal conditions previously studied in our laboratory and to evaluate their pharmacodynamics.Method:Etoposide liposomes were prepared,the encapsulation efficiency and drug loading were evaluated by HPLC.The free Etoposide and drug-loaded liposomes were applied to small cell lung cancer H446 cells and human breast cancer MCF-7 cells and the IC50 was calculated to compare the inhibitory on the two tumor cells.Results:The entrapment efficiency of the prepared drug-loaded nano-liposomes was 79.77%,and the drug loading efficiency was 21.65%.Cell pharmacodynamic evaluation experiments showed that the IC50 values of the free Etoposide and drug-loaded liposomes against H446 cells were 2.40μg/mL and 1.56μg/mL,respectively;the IC50 values of free Etoposide and drug-loaded liposomes against MCF-7 cells were 1.28μg/mL and 0.35μg/mL,respectively.Conclusion:By applying the nanostructured lipid carriers to the model drug Etoposide,the inhibitory of Etoposide on small cell lung cancer H446 cells and human breast cancer MCF-7 cells was significantly enhanced.

关 键 词：依托泊苷 脂质体 抗肿瘤作用 

分 类 号：TQ[化学工程]