血清miR-302b在老年急性心肌梗死中的表达及其临床意义  

Expression of serum miR-302b in elderly patients with acute myocardial infarction and its clinical significance

作　　者：刘肖君[1] 曾敏[1] 符秀虹[1] 何杨利 陈积雄[1] Liu Xiao-jun;Zeng Min;Fu Xiu-hong;He Yang-li;Chen Ji-xiong(Department of Medicine,Hainan Provincial People′s Hospital,Haikou 571127,China)

机构地区：[1]海南省人民医院内科,海南儋州571127

出　　处：《中国急救医学》2019年第7期678-681,共4页Chinese Journal of Critical Care Medicine

基　　金：海南省医药卫生科研基金项目(17A400825 ).

摘　　要：目的探讨血清miR-302b在老年急性心肌梗死(AMI)患者中的表达及其临床意义。方法选取本院收治的138例老年AMI患者和65例老年体检者,比较两组血清miR-302b、心肌肌钙蛋白I(cTnI)及肌酸激酶同工酶(CK-MB)水平变化。应用受试者工作特征(ROC)曲线分析血清miR-302b、cTnI及CK-MB水平对老年AMI的诊断价值。采用Pearson相关性分析,分析老年AMI患者miR-302b水平与cTnI、CK-MB的相关性。结果 AMI组血清miR-302b(4.25±1.30 vs. 0.51±0.16)、cTnI(ng/mL:3.17±1.36 vs. 0.04±0.01)及CK-MB(U/L:73.50±19.36 vs. 11.70±2.25)水平均明显高于对照组(P<0.01)。ROC曲线分析显示,miR-302b、cTnI及CK-MB诊断AMI的临界值分别为2.73、1.52 ng/mL、61.30 U/L,三项联合诊断老年AMI的AUC最大为0.935,95%CI(0.872~0.985),其敏感度和特异度分别为94.8%和89.2%。相关分析显示,老年AMI患者血清miR-302b水平与cTnI、CK-MB均呈正相关(r=0.826、r=0.745,P<0.01)。结论血清miR-302b在老年AMI患者中呈高表达,与cTnI、CK-MB联合检测有助于提高老年AMI诊断的准确性。Objective To investigate the expression of serum miR - 302b in elderly patients with acute myocardial infarction ( AMI) and its clinical significance. Methods A total of 138 elderly AMI patients admitted to our hospital and 65 normal controls were selected to compare the changes of serum miR - 302b, myocardial troponin I ( cTnI) and creatine kinase isoenzyme ( CK - MB ) levels between the two groups. The value of serum miR - 302b, cTnI and CK - MB levels in the diagnosis of AMI in elderly patients was analyzed by ROC curve. Pearson correlation was used to analyze the correlation between the levels of miR - 302b and cTnI, CK - MB in elderly patients with AMI. Results The levels of serum miR - 302b (4. 25 ± 1.30 vs. 0. 51 ± 0. 16), cTnI (3. 17 ± 1.36 vs. 0. 04 ± 0.01, ng/mL) and CK - MB (73. 50 ± 19. 36 vs. 11. 70 ±2. 25, U/L) in the AMI group were significantly higher than those in the control group ( P < 0. 01 ). ROC curve analysis showed that the critical values of miR - 302b, cTnI and CK - MB in the diagnosis of elderly AMI were 2. 73 , 1.52 ng/ mL, 61.30 U/L, respectively. The AUC [ 0. 935 , 95% Cl (0. 872 - 0. 985 )] of three combined diagnosis of elderly AMI was the largest, with sensitivity and specificity of 94. 8% and 89. 2%. Relevant analysis showed that serum levels of miR 一 302b were positively correlated with cTnI and CK - MB in elderly patients with AMI ( r =0. 826, r =0. 745 , P <0. 01 ). Conclusion Serum miR -302b is highly expressed in elderly AMI patients, and the combined detection with cTnI and CK - MB is helpful to improve the accuracy of AMI diagnosis in elderly patients.

关 键 词：急性心肌梗死 miR-302b 诊断 

分 类 号：R542.22[医药卫生—心血管疾病]