Efficacy of tea polyphenols(TP 50) against radiation-induced hematopoietic and biochemical alterations in beagle dogs  

作　　者：Dong Xianzhe Wang Dongxiao Han Jichun Luo Qingliang Guo Daihong Liu Ping Yan Can Hu Yuan 

机构地区：[1]Department of Clinical Pharmacology,General Hospital of Chinese People's Liberation Army,Beijing 100853,China [2]Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China [3]Center of Drug Security, Chinese People's Liberation Army General Hospital, Beijing 100853, China [4]Department of Basic Theory of Chinese Medicine,School of Pre-clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510060, China [5]The Research Centre of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510060, China

出　　处：《Journal of Traditional Chinese Medicine》2019年第3期324-331,共8页中医杂志（英文版）

基　　金：Supported by grants from the National Natural Science Foundation of China:Studies on the Structure of Bioactive Substance from Spatholobus Suberectus Dunn and Its Mechanism of Hematopoietic Regulation Effect(No.30070890);Mechanism of Anti-oxidant Effect and the Activating-hematopoiesis Effect of Spatholobus Suberectus Dunn and Its Active Consititutents(No.81373781)

摘　　要：OBJECTIVE:To investigate the radioprotective ef fect of tea polyphenols(TP 50)against radiation-in duced organ and tissue damage.METHODS:Beagle dogs were exposed to a single acute dose of whole-body y-radiation(3 Gy)and orally administered TP 50(80 or 240 mg·kg-1·d-1)for 28 consecutive days.A hemogram was obtained from experimental dogs every other day for 42 d.At the end of the experiment,enzyme activities of the antioxidants superoxide-dismutase and glutathione peroxidase,serum levels of inflammatory cytokines(tumor necrosis factor-a,interleukin-1β,and interleukin-6),colony-forming units of bone marrow hematopoietic progenitor cells,and organ coefficients were measured.RESULTS:Dogs exposed to γ-radiation alone exhibited typical hematopoietic syndrome.In contrast,irradiated dogs that received TP 50 exhibited an improved blood profile with reduced leucopenia,thrombocytopenia(platelet counts),and reticulocyte levels.TP 50 also significantly elevated levels of the endogenous antioxidant enzyme superoxide-dismutase,reduced the increased levels of serum cytokine in response to radiation-induced toxicity,and increased colony-forming units of bone marrow hematopoietic progenitor cells.In addition,TP 50 repaired radiation-induced organ damage.CONCLUSION:The current findings suggest that oral administration of TP 50 to beagle dogs effectively alleviated hematopoietic bone marrow damage induced by y-radiation.OBJECTIVE:To investigate the radioprotective ef fect of tea polyphenols（TP 50）against radiation-in duced organ and tissue damage.METHODS:Beagle dogs were exposed to a single acute dose of whole-body y-radiation（3 Gy）and orally administered TP 50(80 or 240 mg·kg-1·d-1)for 28 consecutive days.A hemogram was obtained from experimental dogs every other day for 42 d.At the end of the experiment,enzyme activities of the antioxidants superoxide-dismutase and glutathione peroxidase,serum levels of inflammatory cytokines（tumor necrosis factor-a,interleukin-1β,and interleukin-6）,colony-forming units of bone marrow hematopoietic progenitor cells,and organ coefficients were measured.RESULTS:Dogs exposed to γ-radiation alone exhibited typical hematopoietic syndrome.In contrast,irradiated dogs that received TP 50 exhibited an improved blood profile with reduced leucopenia,thrombocytopenia（platelet counts）,and reticulocyte levels.TP 50 also significantly elevated levels of the endogenous antioxidant enzyme superoxide-dismutase,reduced the increased levels of serum cytokine in response to radiation-induced toxicity,and increased colony-forming units of bone marrow hematopoietic progenitor cells.In addition,TP 50 repaired radiation-induced organ damage.CONCLUSION:The current findings suggest that oral administration of TP 50 to beagle dogs effectively alleviated hematopoietic bone marrow damage induced by y-radiation.

关 键 词：Tea POLYPHENOLS Radiation BEAGLE DOGS HEMATOPOIETIC system Cytokines 

分 类 号：R[医药卫生]