An ethanolic extract of Bailian(Radix Ampelopsis Japonicae):demonstration of colorectal cancer treatment efficacy via inhibition of β-catenin signaling in vitro  

作　　者：Su Tao Wang Xinning Li Chunyu Bai Jingxuan Chi-Yan Cheng Fu Xiuqiong Li Ting Yu Zhiling 

机构地区：[1]Consun Chinese Medicines Research Centre for Renal Diseases, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong 999077, China [2]HKBU Shenzhen Research Institute and Continuing Education, Shenzhen 518001, China [3]Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong,999077, China

出　　处：《Journal of Traditional Chinese Medicine》2019年第3期339-345,共7页中医杂志（英文版）

基　　金：Supported by Food and Health Bureau of Hong Kong:Elucidating the Involvement of IL-17-IL-6-STAT3 Axis in the anti-melanoma Effects of a Herbal Formula Comprising Flos Sophorae and Flos Lonicerae(No.HMRF14150571);Science,Technology and Innovation Commission of Shenzhen:anti-colorectal Cancer Effects of Bailian(Radix Ampelopsis Japonicae)extract(No.JCYJ20140807091945050);Exploring the Mechanisms for the anti-rheumatoid Rrthritis Effects of Chinese Medicinal Herb Based on Targeting the TLR4 Signaling Pathway(No.JCYJ20160229210327924);The Research Grants Council of Hong Kong:Elucidating the role of TLR4/STAT3 Signaling in the Antimelanoma Effects of Atractylenolide Ⅱ(No.12125116);The National Natural Science Foundation of China:Exploring the let-7/IGF1R-related Mechanism of Action for the Anti-melanoma Effects of a Herbal formula Huai Hua Jin Yin Jiu(No.81673649);Guangdong Natural Science Foundation:Evaluating the Involvement of miR-34b in the Anti-melanoma Action of Si-Jun-Zi-Tang(No.2016A030313007)

摘　　要：OBJECTIVE:To investigate the underlying mechanism of Bailian (Radix Ampelopsis Japonicae,BL) extract action on colorectal cancer (CRC).METHODS:We explored the involvement of β-catenin signaling on the anti-CRC effects of an BL ethanolic extract (BLE) in cell models by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,immunofluorescent staining,luciferase assay,Western blot analysis and real-time quantitative polymerase chain reaction analysis.Anti-CRC compounds were quantified by high performance liquid chromatography.RESULTS:The contents of gallic acid,catechin,and epicatechin in the BLE were 0.23,1.25,and 0.18 g/kg,respectively.BLE-mediated cytotoxic and apoptotic effects were accompanied by lowered β-catenin/Tcf transcriptional activity,reduced β-catenin nuclear localization,and downregulated protein and mRNA levels of both β-catenin and molecules regulated by β-catenin.CONCLUSION:The mechanism underpinning the anti-CRC effects of BLE may involve inhibition of β-catenin signaling.Further studies are necessary to establish the role of β-catenin signaling in the action of BLE-mediated anti-CRC effects.OBJECTIVE:To investigate the underlying mechanism of Bailian(Radix Ampelopsis Japonicae,BL)ex tract action on colorectal cancer(CRC).METHODS:We explored the involvement of β-catenin signaling on the anti-CRC effects of an BL ethanolic extract(BLE)in cell models by using the 3-(4,5-dim ethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,immunofluorescent staining,luciferase assay,Western blot analysis and real-time quantitative polymerase chain reaction analysis.Anti-CRC compounds were quantified by high performance liquid chromatography.RESU LTS:The contents of gallic acid,catechin,and epicatechin in the BLE were 0.23,1.25,and 0.18 g/kg,respectively.BLE-mediated cytotoxic and apoptotic effects were accompanied by loweredβ-catenin/Tcf transcriptional activity,reducedβ-catenin nuclear localization,and downregulated protein and mRNA levels of both β-catenin and molecules regulated by β-catenin.CONCLUSION:The mechanism underpinning the anti-CRC effects of BLE may involve inhibition ofβ-catenin signaling.Further studies are necessary to esta blish the role of β-catenin sig naling in the action of BLE-mediated anti-CRC effects.

关 键 词：Bailian (Radix Ampelopsis Japonicae) COLORECTAL NEOPLASMS Beta CATENIN CYTOTOXINS Apoptosis 

分 类 号：R285[医药卫生—中药学]