荔枝核总黄酮对肝纤维化大鼠转化生长因子及核因子κB表达的影响  

Effect of total Flavone of Semen Litchi on the expression of transforming growth factor and NF-κB of hepatic fibrosis in rats

作　　者：肖绪华[1] 赵永忠[1] 曹杰 XIAO Xuhua;ZHAO Yongzhongu;CAO Jie(Affiliated Hospital of Guilin Medical College, Guilin 541001;The Second Affiliated Hospital of Guilin Medical College, Guilin 541100, China)

机构地区：[1]桂林医学院附属医院,广西桂林541001 [2]桂林医学院第二附属医院,广西桂林541100

出　　处：《华夏医学》2019年第3期11-15,共5页Acta Medicinae Sinica

基　　金：广西中医药民族医药课题(GZZC16-51)资助

摘　　要：目的:探讨荔枝核总黄酮(TFL)在胆总管结扎诱导的大鼠肝纤维化模型中的治疗效果及其作用机制。方法:将100只雄性SD大鼠随机分为两组:假手术组(SO组,20只)、造模组(80只);造模组采用胆总管结扎制备肝纤维化大鼠模型后随机分为4组,每组20只,分别为胆总管结扎(BDL)组及TFL小、中、大剂量组,TFL小、中、大剂量组于胆总管结扎后分别予TFL 100,200,300 mg·kg^-1·d^-1灌胃。4周后处死大鼠,分别检测大鼠血清中透明质酸(HA)、层黏蛋白(LN)及Ⅲ型前胶原(PCⅢ)的水平;HE染色和Masson染色观察大鼠肝组织病理改变;免疫组化检测转化生长因子β1(TGF-β1)及核因子κB(NF-κB)在肝组织内的表达。结果:与胆总管结扎组大鼠比较, TFL大、中、小剂量治疗后大鼠血清中HA、LN和PCⅢ的水平显著降低(P<0.05),肝组织内纤维化程度降低,肝小叶结构趋于正常,同时3组大鼠肝组织中TGF-β1及NF-κB表达明显降低(P<0.05)。结论:TFL能减轻胆总管结扎诱导的肝纤维化大鼠的肝损伤及纤维化程度,其机制可能与抑制肝内TGF-β1及NF-κB表达有关。Objective:To Explore the effect and action mechanism of total Flavone of Semen Litchi(TFL)on rat model of liver fibrosis. Methods:100 male SD rats were randomly divided into two groups: sham operation group(SO group 20), model group(80). Bile duct occlusive fibrosis model was prepared by bile duct ligation. Model group were randomly divided into four groups on average: bile duct ligation group(BDL group), low-dose TFL group(100 mg· kg^-1· d^-1), middle-dose TFL group(200 mg· kg^-1 · d^-1), hig-dose TFL group(300 mg· kg^-1· d^-1). Three groups of rats were given corresponding doses of TFL by gavage method. After 4-week treatment, the rats were killed. The serum level of hyaluronic acid(HA),laminin(LN),Ⅲ procollagen(PCⅢ)were determined, and the liver histopathological changes were observed by light microscopy after HE and Massonstaining. The expression of transforming growth factor-β1(TGF-β1)and NF-κB in liver tissue was observed by immunohistochemical staining. Results: As compared with BDL group, the serum levels of HA、LN and PCⅢ were significantly decreased(P< 0.05), and the liver fibrosis was significantly improved in the low-dose TFL group, middle-dose TFL group and high-dose TFL group. The expressions of NF-κB and TGF-β1 were decreased in the liver of the rats which were given TFL treatment(P<0.05). Conclusion: TFL may play a protective role in liver injury, which can inhibit liver fibrosis induced by bile duct ligation. The mechanism may be attributed to its effect of down regulating NF-κB and TGF-β1.

关 键 词：荔枝核总黄酮 肝纤维化 转化生长因子 核因子ΚB 

分 类 号：R965[医药卫生—药理学]