Meis1在心肌肥厚中的负性作用  

The negative effect of Meisl on the cardiac hypertrophy

作　　者：张韫佼[1] 梅举[1] 董莉亚[1] 许喜乐 黄雅筠 马南[1] ZHANG Yun-jiao;MEI Ju;DONG Li-ya;XU Xi-le;HUANG Ya-yun;MA Nan(Department of CardiothoracicSurgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092,China)

机构地区：[1]上海交通大学医学院附属新华医院心胸外科,上海市200092

出　　处：《中国心血管病研究》2019年第7期666-670,共5页Chinese Journal of Cardiovascular Research

基　　金：上海市科委科研计划项目(15411952600).

摘　　要：目的通过检测来自肥厚型心肌病患者、小鼠心肌肥厚模型和体外诱导心肌细胞肥大的标本,探讨Meis1在心肌肥厚发生过程中的作用.方法2015年1月至2018年6月,分别获取肥厚型心肌病患者、小鼠心肌肥厚模型和体外诱导心肌的标本,以室壁瘤患者、小鼠假手术组和加入生理盐水培养的心肌细胞为对照组,通过Real-time PCR检测心肌肥厚标志物Nppa和β-MHC(Myh7) mRNA水平,同时检测Meis1mRNA水平.Western blot方法检测Nppa、β-MHC及Meis1的蛋白水平.比较分析与对照组之间的差异.结果三种模型来源的标本中Nppa和Myh7的mRNA和蛋白表达均明显增加(P<0.05),同时Meis1的mRNA和蛋白水平则低于对照组(P<0.05).Meis1与Nppa、Myh7表达的变化呈相反趋势,表明Meis1在心肌肥厚的发生过程中起着负性调节作用.结论 Meis1在心肌肥厚发生过程中起着负性调节作用,上调Meis1表达可能会抑制心肌肥厚的发展.Objective To explore the role of Meisl in the development of cardiac hypertrophy through the samples from hypertrophic cardiomyopathy patients, mouse cardiac hypertrophy model and in vitro induction of cardiomyocyte hypertrophy. Methods From January 2015 to June 2018 Samples from hypertrophic cardiomyopathy patients, mouse hypertrophy model and myocardium in vitro were harvested. Patients with ventricular aneurysm, mouse sham operation group and cardiomyocytes cultured with normal saline were used as control group. The mRNA levels of Nppa and P-MHC(Myh7) were detected by Real-time PCR;and the level of Meisl mRNA was detected. The protein levels of Nppa, p-MHC and Meisl were detected by Western blot. The expression difference was compared to the control group. Results The mRNA and protein expressions of Nppa and Myh7 were significantly increased in the three models(P<0.05), while the mRNA and protein levels of Meisl were lower than those in the control group(P<0.05). Meisl showed opposite trends in the expression to Nppa and Myh7, indicating that Meisl plays a negative regulatory role in the development of cardiac hypertrophy. Conclusion Meisl would be a negative factor for the development of cardiac hypertrophy. Upregulation of Meisl may be able to inhibit the development of cardiac hypertrophy.

关 键 词：Meis1 心肌肥厚 Nppa Β-MHC 

分 类 号：Q95-33[生物学—动物学] R542.22[医药卫生—心血管疾病]