乙酰丙酸-十二烷基硫酸钠对结核分枝杆菌的杀灭作用及其安全性毒理学评价  

Evaluation of Disinfection Efficacy of Levulinic Acid plus Sodium Dodecyl Sulfate for Mycobacterium Tuberculosis and Safety Assessment in Toxicology

作　　者：吕霞丽 李自慧[1] 潘丽萍[1] 贾红彦[1] 张宗德[1] 刘洋[1] Lyu Xiali;Li Zihui;Pan Liping;Jia Hongyan;Zhang Zongde;Liu Yang(Beijing Chest Hospital,Capital Medical University,Beijing Tuberculosis and Thoracic Tumor Research Institute,Beijing Key Laboratory for Drug Resistant Tuberculosis Research,Beijing 101149,China)

机构地区：[1]首都医科大学附属北京胸科医院北京市结核病胸部肿瘤研究所耐药结核病研究北京市重点实验室,101149

出　　处：《结核病与胸部肿瘤》2019年第2期121-126,共6页Tuberculosis and Thoracic Tumor

基　　金：北京市科技新星计划(Z161100004916080);北京市卫生系统高层次卫生技术人才-学科骨干(2015-3-097 );北京市通州区科技计划(KJ2017CX076)。

摘　　要：目的研究消毒剂乙酰丙酸-十二烷基硫酸钠(levulinic acid plus sodium dodecyl sulfate,LVA-SDS)对结核分枝杆菌标准株H37Rv、耐多药结核分枝杆菌临床分离株的杀灭作用;并对LVA-SDS进行安全性毒理学评价。方法按照2002年版卫生部《消毒技术规范》要求,采用悬液定量杀菌试验和动物实验方法,分别评估LVA-SDS对结核分枝杆菌标准株H37Rv、耐多药结核分枝杆菌临床分离株菌悬液的杀灭作用以及LVA-SDS实际使用时的安全性。结果20%(体积分数)LVA+2%(质量分数)SDS消毒剂与1mg/mL的菌悬液作用30min,对菌悬液内结核分枝杆菌标准株H37Rv或耐多药结核分枝杆菌临床分离株的平均杀灭对数值均>5.00。小鼠急性经口毒性试验结果为半数致死量(median lethal dose,LD50)>5000mg/kg,蓄积系数K>5,皮肤刺激反应无红斑和水肿形成,皮肤刺激指数<0.5。结论LVA-SDS对结核分枝杆菌标准株H37Rv、耐多药结核分枝杆菌临床分离株均有较好的杀灭作用;LVA-SDS属实际无毒级、轻度蓄积毒性作用且对皮肤无刺激性。因此,LVA-SDS是一种安全的、对结核分枝杆菌杀灭作用较好的消毒剂,具有较强的实际应用价值。Objective To investigate the germicidal efficacy of levulinic acid plus sodium dodecyl sulfate (LVA-SDS) for H37Rv strain and multidrug-resistant Mycobacterium tuberculosis clinical isolates and to assess the toxicologic safety of LVA-SDS in practical application.Methods According to the 2002 edition of the Technical Specification for Disinfection,suspension quantitative germicidal test and animal experiment methods were respectively used to evaluate the germicidal efficacy of LVA-SDS for H37Rv and multidrug-resistant Mycobacterium tuberculosis isolates and the toxicologic safety of LVA-SDS.Results The average killing logarithm values of 20%LVA+2%SDS for H37Rv or multidrug-resistant Mycobacterium tuberculosis isolates were respectively>5.00.The maximum of the inactivation of the disinfectant on the acute toxicity test was LD50>5 000 mg ·kg^-1.Cumulative coefficient K>5.No erythema and edema were found in skin irritation,and the skin irritation index was<0.5.Conclusion LVA-SDS has high efficacy for killing H37Rv and multidrug-resistant Mycobacterium tuberculosis isolates with actually non-toxic grade,and no cumulative toxicity and no skin stimulation.Therefore,LVA-SDS is a safe disinfectant for Mycobacterium tuberculosisand has strong practical application value.

关 键 词：结核分枝杆菌 乙酰丙酸(LVA) 平均杀灭对数值 安全性 

分 类 号：R378.911[医药卫生—病原生物学]