肠内营养联合益生菌对再生障碍性贫血大鼠炎症因子和免疫功能的影响  

Effect of enteral nutrition and probiotics on inflammatory factors and immune function in aplastic anemia rats

作　　者：王毅[1] 单培仁[1] 黄伟[1] Wang Yi;Shan Peiren;Huang Wei(Department of nutrition,the First Hospital Affiliated to Wenzhou Medical College,Wenzhou 325000,China)

机构地区：[1]温州医科大学附属第一医院营养科,温州325000

出　　处：《中华临床营养杂志》2019年第3期179-183,共5页Chinese Journal of Clinical Nutrition

基　　金：浙江省自然科学基金项目(LQ15H020005).

摘　　要：目的探讨肠内营养联合益生菌对再生障碍性贫血(AA)大鼠炎症因子和免疫功能的影响方法采用腹腔注射氟尿嗜噪联合灌胃马利兰法建立AA大鼠模型,随机分为模型组、肠内营养组、益生菌组和联合组,每组10只,另选10只Wistar大鼠作为空白组,4周模型成功后,空白组和模型组给予生理盐水灌胃(等体积0.9%NaCl),肠内营养组给予百普素营养液灌胃[10g/(kg·d)],益生菌组给予酪酸梭菌活菌灌胃[2g/(kg·d)],联合组给予百普素[10g/kg·d)和酪酸梭菌活菌[10g/(kg·d)]灌胃,连续灌胃4周,HE染色观察骨髓病理结构,血细胞分析仪检测白细胞(WBC)、红细胞(RBC)、血小板(PLT)、血红蛋白(Hb)含量,ELISA法检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)和白细胞介素-11(IL-11)水平,流式细胞仪检测CD4^+、CD8^+细胞及NKT细胞含量。结果模型组WBC、RBC、PLT和Hb含量低于空白组,肠内营养组、益生菌组和联合组WBC、RBC、PLT和Hb含量高于模型组,且联合组优于肠内营养组和益生菌组,差异均有统计学意义(P<0.05),肠内营养组和益生菌组比较,差异无统计学意义(P>0.05)。模型组TNF-α、IL-2水平高于空白组,而IL-11低于空白组,肠内营养组、益生菌组和联合组TNF-α、IL-2水平低于模型组,而IL-11高于模型组,且联合组优于肠内营养组和益生菌组,差异均有统计学意义(P<0.05),肠内营养组和益生菌组比较,差异无统计学意义(P>0.05)。模型组CD4^+和NKT细胞低于空白组,CD8^+高于空白组,肠内营养组、益生菌组和联合组CD4^+和NKT细胞含量高于模型组,CD8^+含量低于模型组,且联合组优于肠内营养组和益生菌组,差异均有统计学意义(P<0.05),肠内营养组和益生菌组比较,差异无统计学意义(P>0.05)。结论与普通进食或口服葡萄糖相比,肠内营养联合益生菌可减轻再生障碍性贫血大鼠炎症反应,改善骨髓造血功能,提高免疫功能。Objective To investigate the effect of enteral nutrition and probiotics on inflammatory factors and immune function in aplastic anemia ( AA) rats. Methods AA rats model were established by intraperitoneal injection of fluorouracil and lavage Maryland, and the successful AA rats model were randomly divided into model group, enteral nutrition group, probiotics group and combination group, 10 rats in each group. Another 10 Wistar rats were selected as blank group. Rats in the blank group and model group were given saline lavage ( same volume 0.9% NaCl), rats in the enteral nutrition group were given Peptisorb nutrient solution [10 g/(kg·d)] by lavage, rats in the probiotics group were given live Clostridium butyricum [ 2 g/( kg·d)] by lavage, and rats in the combination group were given Peptisorb nutrient solution [ 10 g/( kg ·d)] and live Clostridium butyricum [2 g/(kg·d)] by lavage for 4 weeks. The pathological structure of the bone marrow were observed by HE staining, the contents of white blood cells ( WBC ), red blood cell ( RBC ), platelet (PLT) and hemoglobin ( Hb) in blood cell were detected by analyzer, the levels of serum tumor necrosis factor alpha (TNF-α), interleukin-2 ( IL-2) and interleukin-11 (IL-11) were detected by ELISA method, and the contents of CD4^+, CD8^+ and NKT cells were detected by flow cytometry instrument. Results The contents of WBC, RBC, PLT and Hb in the model group were significantly lower than that in the blank group, the contents of WBC, RBC, PLT and Hb in enteral nutrition group, probiotics group and combination group were significantly higher than that in the model group, and the combination group was better than the enteral nutrition group and probiotics group ( P<0.05), but the enteral nutrition group and probiotics group had no statistically significant difference (P>0.05). The level of TNF-α and IL-2 were significantly higher and the IL-11 level was significantly lower in the model group than in the blank group, the level of TNF-α and IL-2 were significantly lower and

关 键 词：再生障碍性贫血 肠内营养 益生菌 炎症因子 免疫功能 

分 类 号：R556.5[医药卫生—血液循环系统疾病]