广东汉族人群ADIPOQ基因rs266729位点单核甘酸多态性与2型糖尿病遗传易感性研究  

Relationship between ADIPOQ rs2667292 single nucleotide polymorphism and genetic susceptibility to type 2 diabetes mellitus in Guangdong Han population

作　　者：于海兵 闫妍 巫舢 何小艺 胡维 潘海燕 孔丹莉 林子牛 朱萧 丁元林 YU Haibing;YAN Yan;WU Shan;ZHU Xiao;DING Yuanlin(Department of Epidemiology and Medical Statistics,Guangdong Medical University,Dongguan 523808,China)

机构地区：[1]广东医科大学流行病与卫生统计学教研室 [2]深圳中迈数字医疗技术有限公司

出　　处：《中国糖尿病杂志》2019年第7期481-485,共5页Chinese Journal of Diabetes

基　　金：国家自然科学面上基金(81273166);广东省自然科学基金(2016A030313674);2018年度广东省医学科研基金项目(B2018074);2018年度广东省科技发展专项资金(基础与应用基础研究方向)博士科研启动(2018A030310115);2018年广东省大学生创新训练项目(201810571102);2017年广东高校省级重点平台和重大科研特色创新类项目(2017KTSCX084);2018年东莞市社会科技发展(一般)项目(20185071521339);深圳市技术创新计划技术攻关项目(JSGG2010331103247408);东莞市社会科技发展项目(2016108101028)

摘　　要：目的探讨广东地区汉族人群内ADIPOQ基因内rs266729位点与T2DM的遗传易感性的关系。方法采用病例对照研究方案,通过SNPscan TM单核苷酸多态性(SNP)分型技术检测T2DM患者(T2DM组)和正常对照组(NC组)ADIPOQ基因内rs266729位点的基因型。将问卷信息、一般资料及生化指标结果与SNP数据整合,进行数据处理,并对基因型及等位基因频率在两组中的分布进行分析。结果单位点基因关联分析发现,ADIPOQ基因内rs2667292位点满足Hardy-Weinberg平衡。ADIPOQ基因rs266729位点C、G等位基因在T2DM组与NC组的分布依次为74.1%、75.1%、25.9%、24.9%,C、G等位基因分布及SNP的基因型分布,差异无统计学意义(P>0.05)。比较遗传模型,调整协变量前后,两组rs266729位点在共显性、显性、隐性及超显性遗传模型下差异均无统计学意义(P>0.05)。结论广东地区汉族人群T2DM患者遗传易感性可能与ADIPOQ基因rs266729位点单核苷酸多态性无关。Objective To investigate the association between single nucleotide polymorphism rs266729 locus in ADIPOQ(Adiponectin)gene and type 2 diabetes mellitus(T2DM)susceptibility in Han population in Guangdong province.Methods Based on a case-control study,the genotype of rs266729 in the ADIPOQ gene was detected by SNPscan TM typing technique. The verified questionnaire information,physical examination and clinical biochemical examination Results were integrated with SNP data. SPSS 20. 0 statistical software package was used for data processing. The distribution of genotype and allele frequency between the two groups was analyzed.Results The single locus association analysis found that the rs2667292 locus in the ADIPOQ gene satisfies the Hardy-Weinberg equilibrium. The distribution of C and G alleles on ADIPOQ rs266729 in T2DM group and control group were 74. 1%,75. 1%,25. 9% and24. 9% in sequence(P>0. 05). Before and after adjusting the covariates,there was no significant difference in the distribution of rs266729 locus between the two groups in the co-dominant,dominant,recessive or super-dominance genetic model(P>0. 05).Conclusion Genetic susceptibility to T2DM may be independent of the single nucleotide polymorphism of the ADIPOQ gene rs266729 locus in Guangdong Han population.

关 键 词：糖尿病 2型 ADIPOQ基因 rs266729 单核苷酸多态性 

分 类 号：R587.1[医药卫生—内分泌]