针灸对糖尿病造影剂肾病大鼠肾脏细胞凋亡基因Fas/FasL的影响  

Synergistic effect of acupuncture and moxibustion on expression of renal Fas and FasL mRNAs and proteins in contrast-induced nephropathy diabetic rats

作　　者：高巧营[1] 赵凯[2] 杨秋汇[3] 陈卓阳 宗春辉[1] GAO Qiao-ying;ZHAO Kai;YANG Qiu-hui;CHEN Zhuo-yang;ZONG Chun-hui(Institute of Acute Abdomen of Integration of Chinese and Western Medicine, Tianjin 300100, China;Department of Cardiology,Tianjin Nankai Hospital, Tianjin 300100;Department of Acupuncture and Moxibustion. Tianjin Nankai Hospital, Tianjin 300100)

机构地区：[1]天津市中西医结合急腹症研究所,天津300100 [2]天津市南开医院心血管内科,天津300100 [3]天津市南开医院针灸科,天津300100

出　　处：《针刺研究》2019年第7期469-475,共7页Acupuncture Research

基　　金：国家自然科学基金项目(No.81503409);天津市卫生局科技基金项目(No.2014KZ050)

摘　　要：目的:通过观察针灸对糖尿病造影剂肾病大鼠肾脏细胞凋亡基因Fas/FasL的影响,探讨针灸对糖尿病造影剂肾病的保护机制。方法:雄性SD大鼠随机分为对照组、模型组、针刺组、艾灸组、联合组,每组8只。采用腹腔注射链脲佐菌素法制备糖尿病造影剂肾病大鼠模型。针刺组、艾灸组及联合组分别给予针刺、艾灸及针刺+艾灸联合治疗,3组均选取"三阴交""肾俞""脾俞",每日1次,连续7d。用HE染色法观察大鼠肾脏病理形态,醋酸铀-柠檬酸双染色法观察大鼠肾脏超微结构,脲酶法测定大鼠血清尿素氮(BUN)含量,苦味酸法测定大鼠血清肌酐(Scr)含量,氧化还原法检测大鼠肾脏组织一氧化氮合酶(NOS)活性,黄嘌呤氧化酶法检测大鼠肾脏组织超氧化物歧化酶(SOD)活性,硫代巴比妥酸法检测大鼠肾脏组织丙二醛(MDA)含量,比色法检测大鼠肾脏组织总抗氧化能力(T-AOC),Western blot法及荧光定量PCR法检测大鼠肾脏组织Fas、FasL蛋白及mRNA表达水平。结果:光学显微镜下,对照组大鼠肾小球、肾小管及间质无明显病理改变;模型组大鼠肾小球呈分叶状,肾小管上皮细胞多有局部坏死、空泡样变性。电镜下模型组大鼠肾小球血管袢基底膜增厚,肾小管上皮细胞胞质见大量空泡,线粒体严重肿胀,线粒体嵴排列紊乱、断裂或消失,可见凋亡样改变的细胞。针刺组和艾灸组肾脏病理改变基本同模型组;联合组大鼠肾脏病理改变有所改善。与对照组比较,模型组大鼠血清BUN、Scr含量明显升高(P<0.01);与模型组比较,艾灸组大鼠血清BUN含量明显降低(P<0.05),针刺组、联合组大鼠血清BUN、Scr含量明显降低(P<0.05);与针刺组比较,联合组大鼠血清BUN含量明显降低(P<0.05);与艾灸组比较,联合组大鼠血清BUN、Scr含量明显降低(P<0.05)。与对照组比较,模型组大鼠肾脏NOS、SOD、T-AOC活性明显降低(P<0.05)、MDA含量明显升高(P<0.01);与模�Objective To observe the effect of acupuncture and moxibustion on renal Fas and Fas ligand(FasL expression in contrast-induced nephropathy(CIN)diabetic rats,so as to explore its protective mechanisms.Methods A total of 40 diabetic male SD rats were randomly divided into control,model,acupuncture,moxibustion and combined treatment(acupuncture combined with moxibustion)groups(n=8 rats in each group).The diabetic model was established by i.p.injection of Streptozotocin(60 mg/kg),followed by regular raising for 9 weeks.Then,the manual acupuncture,moxibustion or acupuncture plus moxibustion at "Sanyinjiao"(SP6)"Shenshu"(BL23)and"Pishu"(BL20)was given to the diabetic rats,once daily for 7 days.On the 8 th day,these diabetic rats received i.p.injection of compound Meglumine Diatrizoate Injection(10 mL/kg)for establishing CIN model.Then,the venous blood specimens were collected for detecting contents of serum urine nitrogen(BUN,with urease method)and creatinine(Scr,with picric acid method).The kindey tissues were exteriorized for detecting nitricoxide synthase(NOS,with oxidation-reduction method),malondialdehyde(MDA,with thiobarbituric acid method),superoxide dismutase(SOD,with xanthine oxidase technique)and total antioxidant capacity(T-AOC,with colorimetric technique).The expression levels of Fas and FasL in the kidney tissues were detected by quantitative real-time PCR and Western blot separately.The histopathological and ultrastructural changes of the kidney were observed under microscope(after H.E.staining)and transmission electron microscope,respectively.Results Following modeling and compared with the control group,the BUN,Scr and renal MDA contents,and Fas and FasL mRNA and protein expression levels were significantly increased(P<0.01,P<0.05),and renal NOS,SOD and T-AOC levels considerably decreased in the model group(P<0.05).After the interventions and compared with the model group,serum BUN and Scr contents and renal Fas mRNA and protein expression levels in both acupuncture group and combined treatment group,BU

关 键 词：针灸 造影剂肾病 糖尿病 FAS/FASL 

分 类 号：R245.3[医药卫生—针灸推拿学]