电针对腹型肥胖大鼠肝脏脂代谢及Sirt1/PPARγ通路的影响  

Impact of electroacupuncture on liver lipid metabolism and hepatic Sirt1 and PPARγ expression in abdominal obese rats

作　　者：王海英 方红娟 王强 梁翠梅[1] 胡慧[1] WANG Hai-ying;FANG Hong-juan;WANG Qiang;LIANG Cui-mei;HU Hui(Department of Acupuncture-moxibustion, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China;Department of Endocrinology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070;Institute of Disease Control and Prevention of Chinese People's Liberation Army, Beijing 100071)

机构地区：[1]北京中医药大学东方医院针灸科,北京100078 [2]首都医科大学附属天坛医院内分泌科,北京100070 [3]中国人民解放军疾病预防控制中心,北京100071

出　　处：《针刺研究》2019年第7期492-496,511,共6页Acupuncture Research

基　　金：北京市科学技术委员会“首都临床特色应用研究”专项课题(Z141107002514079);北京市中医管理局中医药“3+3”薪火传承工程《杨甲三名家研究室》;北京市医管局青苗人才计划资助项目(No.QML20160501)

摘　　要：目的:探讨电针对高脂饮食诱导的腹型肥胖大鼠肝脏脂代谢及沉默信息调节因子1(Sirts1)、过氧化物酶体增殖物激活受体γ(PPARγ)表达的影响。方法:SD雄性大鼠,随机分为空白组6只,造模组29只。造模组大鼠高脂饮食喂养12周,建立腹型肥胖模型。造模成功的12只腹型肥胖大鼠,随机分为模型组6只、针刺组6只,针刺组大鼠电针双侧"带脉"穴,2Hz/15Hz疏密波,电流强度1.5mA,每次20min,隔日1次,干预8周。每周测量体质量、腹围。针刺8周末,采用全自动生化仪检测血清总胆固醇(TC)、甘油三酯(TG)、谷丙转氨酶(ALT)、谷草转氨酶(AST),油红"O"染色法观察肝脏病理形态学变化,荧光定量PCR法检测肝组织Sirt1和PPARγ mRNA的表达,Western blot法检测肝组织Sirt1和PPARγ蛋白的表达水平。结果:与正常组比较,模型组的体质量、腹围明显增加(P<0.001,P<0.01),血清TC、TG、ALT、AST含量明显升高(P<0.01),肝脏脂质堆积明显增加,Sirt1 mRNA及蛋白的表达明显下降(P<0.05,P<0.01),PPARγ mRNA及蛋白的表达明显增加(P<0.01,P<0.05);与模型组比较,针刺组大鼠体质量、腹围明显减小(P<0.05,P<0.01),TC、TG、ALT、AST明显降低(P<0.05),肝脏脂质堆积明显减轻,肝组织Sirt1 mRNA及蛋白的表达显著增加(P<0.001,P<0.05),PPARγ mRNA及蛋白的表达明显降低(P<0.05,P<0.01)。结论:针刺可改善腹型肥胖大鼠肝脏脂质代谢,其机制可能与上调Sirt1、下调PPARγ的表达相关。Objective To observe the impact of electroacupuncture(EA)on liver lipid metabolism and expression of hepatic sirtuin 1(Sirt1)and peroxisome proliferator activated receptorγ(PPARγ)of abdominal obese rats induced by high-fat diet.Methods Eighteen male SD rats were divided into blank control,model and EA groups(n=6 per group).The abdominal obesity model was established by feeding the rats with high-fat diet for 12 weeks.EA(2 Hz/15 Hz,1.5 mA)was applied to bilateral"Daimai"(GB26)for 20 min every time,once every other day for 8 weeks.Rats of the model group were also restrained for 20 min.The body mass and abdominal circumference were measured every week,and the contents of serum cholesterol(TC),triglyceride(TG),alanine transaminase(ALT),aspartate aminotransferase(AST)were detected by using an automated biochemical analyzer.Histopathological changes of the liver tissues were observed under microscope after oil red "O"staining.The expression of hepatic Sirt1 and PPARγ mRNAs and proteins were detected using quantitative real time PCR and Western blot,separately.Results After modeling,the body weight and abdominal circumference,and serum TC,TG,ALT and AST contents,and expression of hepatic PPARγ mRNA and protein were significantly increased(P<0.001,P<0.01,P<0.05),and the expression levels of hepatic Sirt1 mRNA and protein obviously down-regulated(P <0.05,P <0.01)in the model group.Following EA intervention,the increased body weight and abdominal circumference,and serum TC,TG,ALT and AST contents,and hepatic PPARγ mRNA and protein expression were remarkably suppressed(P<0.05,P<0.01),and the decreased hepatic Sirt1 mRNA and protein were remarkably up-regulated(P<0.001,P<0.05).The lipid droplets in hepatocytes were reduced in the EA group relevant to the model group.Conclusion EA intervention can significantly improve the liver lipid metabolism of abdominal obese rats,which is possibly related with its effect in up-regulating the expression of hepatic Sirt1 mRNA and protein,and in down-regulating the expression of hepa

关 键 词：电针 腹型肥胖 脂代谢 沉默信息调节因子1 过氧化物酶增殖物激活受体Γ 

分 类 号：R245.97[医药卫生—针灸推拿学]